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Master's Dissertation
DOI
Document
Author
Full name
Maeli Melotto
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 1993
Supervisor
Title in Portuguese
Eficiência simbiótica na fixação de nitrogênio em soja e caupi inoculados com as estirpes SR e Semia-587 de Bradyrhizobium japonicum
Keywords in Portuguese
CAUPI
FIXACAO SIMBIOTICA DE NITROGENIO
RIZOBACTÉRIAS
SOJA
Abstract in Portuguese
As possíveis relações entre características fisiológicas e as enzimas glutamina sintetase e alantoinase com a fixação biológica de nitrogênio e expressão Hup (hydrogen uptake) foram estudadas neste trabalho. Plantas de caupi (Vigna unguiculata L. Walp) e soja (Glycine max L. Merril) inoculadas com as estirpes SR ( Hup+) e Semia-587 (Hup-hr) de Bradyrnizobium japonicum foram cultivadas em solução nutritiva livre de nitrogênio combinado em casa-de-vegetação. Caupi inoculado com SR, apresentou menor liberação de hidrogênio, peso da matéria fresca de nódulos, atividade de redução de acetileno e alantoinase nas folhas do que quando inoculado com Semia-587. Nos outros parâmetros estudados as estirpes se comportaram de maneira semelhante. Soja inoculada com a estirpe SR apresentou maiores valores de acúmulo de fitomassa e nitrogênio na parte aérea eficiência nodular, atividade específica de redução de acetileno e menor peso de matéria fresca de nódulo s em relação à soja inoculada com a estirpe Semia-587. Em todas as simbioses estudadas a eficiência relativa da nitrogenase esteve ao redor de 0,97, indicando que as diferenças observadas não são consequência direta dos benefícios da hidrogenase. No entanto, é possível que o fenótipo Hup + esteja associado geneticamente, a características fisiológicas que contribuem para a maior eficiência simbiótica. Foi observado que a atividade de glutamina sintetase tem pouca relação com a atividade específica da nitrogenase, indicando a pequena integração destes processos nas simbioses em questão
Title in English
Symbiotic efficiency on nitrogen fixation in soybean and cowpea inoculated with the strains SR and SEMIA-587 of Bradyrhizobium japonicum
Abstract in English
Possible relationships between the physiological characteristcs and the enzymes glutamine synthetase and allantoinase with biological nitrogen fixation and Hup (hydrogen uptake) expression were studied. Cowpea (Vigna unguiculata L. Walp) and soybean (Glycine max L. Merril) plants inoculated with the strains SR (Hup+) and SEMIA-587 (Hup-hr) of Bradyrhizobium japonicum were grown in nitrogen free nutrient solution, under greenhouse conditions. The symbioses cowpea x SEMIA-578 showed higher nitrogenase and allantoinase activities, hidrogen evolution and nodule weigh than cowpea x SEMIA-587. The other parameters studied, showed no statistical differences. Soybean inoculated with strain SR showed higher plant dry weigh, nitrogen content, nodule efficiency, and specific nitrogenase activity than soybean inoculated with strain SEMIA-587. Also, soybean x SR showed lower nodule weigh than soybean x SENIA-587. In the symbioses studied, the relative efficiency of nitrogenase was about 0.97, indicating that differences observed can not be atributed only to differences in hydrogenase activity. However, it is possible that the phenotype Hup+ was associated genetically with physiological characteristcs which contributed to increasing the symbiotic efficiency. It was observed that glutamine synthetase activity has little relationship with specific nitrogenase activity, indicating a lack of interaction between these processes in the symbioses studied.
 
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2019-08-22
 
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