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Master's Dissertation
DOI
10.11606/D.10.2012.tde-02102012-151003
Document
Author
Full name
Patricia Rotta Lopes
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2012
Supervisor
Committee
Oliveira, Claudio Alvarenga de (President)
Crusco, Silvia Edelweiss
Vannucchi, Camila Infantosi
Title in Portuguese
Avaliação da progesterona salivar em cadelas durante o período peri-ovulatório
Keywords in Portuguese
Cadelas
Imunoensaio
Progesterona
Reprodução
Saliva
Abstract in Portuguese
Vários autores já enfatizaram a importância do monitoramento do ciclo estral em cadelas e citaram exemplos de como ele pode ser feito. O objetivo deste estudo foi avaliar a técnica de dosagem de progesterona salivar para monitorar o ciclo estral da espécie. Para composição do grupo experimental, foram utilizadas 13 cadelas. As amostras de sangue e saliva foram colhidas paralelamente em todos os animais, a partir dos primeiros sinais de proestro. As amostras salivares foram obtidas com o uso de dispositivo específico para coleta Salivette®, método que se mostrou eficaz, visto que foi possível obter volume suficiente para dosagem de progesterona na grande maioria das amostras. As concentrações de progesterona no soro foram determinadas pela técnica de RIE e na saliva por EIE. Embora haja uma relação linear crescente e positiva entre a progesterona sérica e salivar (r=0,704; p<0,0001), não é possível utilizar o parâmetro salivar para determinar o momento da ovulação.
Title in English
Evaluation of salivary progesterone in bitches during periovulatory period
Keywords in English
Bitches
Immunoassay
Progesterone
Reproduction
Saliva
Abstract in English
Several authors have already emphasized the importance of monitoring estrous cycle in bitches and mentioned examples of how it can be done. The aim of this study was to evaluate the salivary progesterone quantification technique in order to monitor the estrous cycle in this species. To compound the experimental group, 13 bitches were used. Blood and saliva samples were collected simultaneously in all animals, starting about the first day of proestrus signs. Salivary samples were collected with a specific device: Salivette®. This method was effective, since it was possible to obtain enough volume in almost all samples to quantify progesterone. Serum progesterone was quantified by radioimmunoassay and salivary progesterone by enzyme immunoassay. Although there is an increasing, linear and positive correlation between salivary and serum progesterone (r=0,704; p<0,0001), it is not possible to use the salivary parameter to set the moment of ovulation.
 
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Publishing Date
2013-06-04
 
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