Doctoral Thesis
DOI
https://doi.org/10.11606/T.10.2002.tde-04042003-103133
Document
Author
Full name
Maria Amelia Zogno
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2002
Supervisor
Committee
Miglino, Maria Angelica (President)
Barnabe, Valquiria Hyppolito
Guimaraes, Marcelo Alcindo de Barros Vaz
Toniollo, Gilson Hélio
Vicente, Wilter Ricardo Russiano
Title in Portuguese
Aspectos reprodutivos da fêmea de mocó (Kerodon rupestris): análise bioquímica dos líquidos fetais e caracterização colpocitológica do ciclo estral.
Keywords in Portuguese
(Kerodon Rupestris)
bioquímica
ciclo estral animal
citologia vaginal
líquido fetal animal
Abstract in Portuguese
Foram utilizados 10 sistemas genitais femininos de mocós (Kerodon rupestris) para estabelecer parâmetros de referência relativos à osmolaridade, pH, cálcio, fósforo, uréia, creatinina, glicose e proteínas totais. Os animais foram criados em cativeiro no CEMAS(Centro de Criação de Animais Silvestres), Mossoró RN. As fêmeas estavam da metade
(30-45 dias) para o final da gestação (65-70 dias). As bolsas amnióticas e alantoideanas
foram puncionadas individualmente para colheita dos líquidos fetais, que foram
centrifugados e analisados posteriormente. Para o líquido amniótico, as concentrações
médias em mg/dl foram: glicose = 45,87 ± 22,38; cálcio = 6,31 ± 1,24; fósforo = 1,64 ±
0,72; creatinina = 0,45 ± 0,12; uréia = 34,03 ± 5,96; proteínas totais = 31,24 ± 16,67.
Para o líquido alantoideano, as concentrações médias em mg/dl foram: glicose = 59,17 ±
10,85; cálcio = 5,58 ± 0,59; fósforo = 1,27 ± 0,73; creatinina = 0,38 ± 0,38; uréia =
31,49 ± 2,28; proteínas totais = 30,70 ± 18,39. Foram observadas pequenas oscilações
entre as concentrações dos parâmetros bioquímicos do fluido amniótico e alantoideano.
Estas concentrações são determinadas, provavelmente, pela atividade metabólica
materno-fetal. Para os estudos colpocitológicos foram utilizadas 8 fêmeas, sexualmente
maduras, mantidas em cativeiro sob condições climáticas tropicais. Foram realizados
esfregaços vaginais a cada 72 horas, durante 40 dias. Os animais mostraram ciclo
poliestral contínuo, com duração média de 24,25 ± 3,99 dias, não havendo portanto
sazonalidade. Durante o proestro houve prevalência de células superficiais anucleadas,
intermediárias e parabasais. A fase de estro apresentou índices significativamente mais
altos de células superficiais anucleadas e ausência de leucócitos. Alto índice de células
intermediárias foi observado no metaestro, com abundância de leucócitos e vacúolos
citoplasmáticos (células de Foam). O anestro mostrou índices significativamente mais
altos de células parabasais quando comparados com as outras fases do ciclo. Os
leucócitos apareceram em número reduzido.
Title in English
Reproductive aspects of the mocó female (Kerodon rupestris): fetal fluids biochemistry and characterization of the estrous cicle by vaginal cytology.
Keywords in English
biochemistry
estrous cycle
fetal fluids
Kerodon rupestris
vaginal cytology.
Abstract in English
The aim of the present work was to study some reproductives aspects of mocó female
(Kerodon rupestris), by assesment of the following parameters: certain biochemical
characteristics of fetal fluids and characterization of the estrous cycle by exfoliative
colpocytology. In order to establish the levels of the following parameters: osmolarity,
pH, calcium, phosphorous, total protein, urea, creatinina, glycose, presents in the
amniotic and allantoic fetal fluids, 10 genital systems of mocós were sampled (gestation
= 30-45 and 65-70 days). The amniotic and allantoic sacs were punctured and the fetal
fluid obtained for posterior analysis. It was observed the following concentrations
(mg/ml) in the amniotic fluid: glycose= 45,87 ± 22,38; calcium = 6,31 ± 1,24;
phosphorus = 1,64 ± 0,72; creatinine = 0,45 ± 0,12; urea = 34,03 ± 5,96; total protein
= 31,24 ± 16,67. In the allantoic fluid the concentrations were: : glycose = 59,17 ±
10,85; calcium = 5,58 ± 0,59; phosphorous = 1,27 ± 0,73; creatine = 0,38 ± 0,38; urea
= 31,49 ± 2,28; total protein = 30,70 ± 18,39. Results obtained from the analisys of the
composition of fetal fluids showed small oscillations between the amniotic and the
allantoic fluids. Their concentrations, probably, are determined by the materno-fetal
metabolic activity. Colpocytology studies were realized using 8 adult females kept in
captivity, in climatologic conditions of humid tropic. Vaginal smears, prepared by means
of swabs, were made each 72 hours, during 40 days. Animals showed a polyestrus
continuos cycle (on an average 24,25 ± 3,99 days), so no sazonality was observed.
During proestrus phase the exfoliative arrangement showed high frequency of anucleated
superficial, intermediate and parabasal cells, respectively. Oestrus phase had elevated
rates of superficial cells (both anucleated and nucleated) and absence of leukocytes. In
the metestrus phase it was found highest indices of intermediate cells and increase of
leukocytes. During the anestrus phase the predominant cells type was parabasal, and
leukocytes were reduced.
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Publishing Date
2004-09-13