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Doctoral Thesis
DOI
https://doi.org/10.11606/T.10.2016.tde-06032015-145258
Document
Author
Full name
Dayane Alcântara
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Miglino, Maria Angélica (President)
Costa, Mirela Tinucci
Flores, Fernanda Faião
Matera, Julia Maria
Nishiyama, Ana Claudia Oliveira Carreira
Title in Portuguese
Interação entre células-tronco de polpa dentária imatura e o osteossarcoma canino
Keywords in Portuguese
Células-tronco de polpa dentária
Células-tronco mesenquimais
Microambiente tumoral
Osteossarcoma
Proliferação celular
Abstract in Portuguese
O osteossarcoma é um tumor ósseo maligno, de maior ocorrência em cães, possui rápido crescimento e alto potencial metastático. Assim, o cão é um modelo útil para o estudo da doença em humanos, tendo em vista as semelhanças clínicas e histopatológicas que ocorrem em ambas às espécies. Atualmente, os estudos a respeito de células-tronco são promissores considerando seu alto potencial terapêutico. Entretanto, ainda prevalecem muitas dúvidas referentes ao tratamento de tumores utilizando a terapia celular. Este tema é pouco conhecido e estudado, por isso, o objetivo deste trabalho foi avaliar a interação entre células-tronco obtidas da polpa dentária canina com as células derivadas osteossarcoma canino. Foram realizados cocultivos celulares das células derivadas de polpa dentária canina, osteossarcoma canino e derivadas de osso normal canino. Analisou-se os aspectos morfológicos das células cocultivadas e controle, assim como a atividade proliferativa, a morte celular, o potencial elétrico mitocondrial e a expressão gênica. A partir dos resultados obtidos, concluiu-se que a interação entre a célula-tronco da polpa dentária canina imatura e as células de osteosarcoma canino não apresentam alterações morfológicas. Entretanto, as células-tronco derivadas da polpa dentária canina e de osso fetal canino sadio parecem servir de suporte para o crescimento tumoral. Além disso, a cocultura celular, em todos os grupos testados, promove alterações na expressão gênica e proteica.
Title in English
Interaction between immature dental pulp stem cells and canine osteosarcoma
Keywords in English
Cell proliferation
Mesenchymal stem cells
Osteosarcoma
Stem cells from dental pulp
Tumor microenvironment
Abstract in English
Osteosarcoma is a malignant bone tumor most frequent in dogs. It has fast growth and high metastatic potential. Thus, the dog is an useful model for the study of human disease, due to the clinical and histological similarities found in both species. Currently, studies about stem cells are promising considering its high therapeutic potential. However, many doubts still exist regarding the treatment of tumors using cell therapy. This theme is little known and studied. Therefore, the aim of this study was to evaluate the interaction between stem cells obtained from canine immature dental pulpstem cells with osteosarcoma cells derived from dogs. Cellular coculture were performed using cells derived from canine dental pulp, canine osteosarcoma and canine normal bone. The morphological aspects of cocultured cells and control were analyzed, as well as proliferative activity, cell death, the mitochondrial membrane electric potential and gene expression. In summary, it was concluded that the interaction between stem cells from canine immature dental pulp and canine osteosarcoma cells did not show morphological changes. However, stem cells derived from canine dental pulp and healthy canine fetal bone serve to support tumor growth. Furthermore, the cell coculture in all groups tested, causes changes in gene and protein expression.
 
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Publishing Date
2016-03-18
 
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