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Master's Dissertation
DOI
https://doi.org/10.11606/D.10.2024.tde-21062024-162915
Document
Author
Full name
Renata Pamela Barrachini Steffen
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2024
Supervisor
Committee
Knöbl, Terezinha (President)
Flores, Fernanda
Moreno, Andrea Micke
Title in Portuguese
Caracterização fenotípica e genotípica de Escherichia coli isolada de pintainhos e embriões no incubatório
Keywords in Portuguese
Antibiograma
APEC
Escherichia coli
Incubatório
MLST
Abstract in Portuguese
O Brasil se destaca a cada ano pelo aumento da produção avícola, e um elo muito importante dentro da cadeia produtiva é o incubatório. A incubação tem um impacto significativo na vida e qualidade dos pintainhos, e é fundamental para que as empresas sejam produtivas e competitivas no mercado. No entanto, algumas enfermidades podem comprometer a viabilidade das aves na fase embrionária, com impacto na saúde animal e na saúde pública. A onfalite, que tem Escherichia coli (E. coli) como principal agente etiológico, é uma enfermidade que acomete pintainhos recém-nascidos até o 7º. dia de vida, causando alta mortalidade e diminuição de desempenho nos pintinhos assintomáticos. Este estudo teve por objetivo determinar a frequência de ocorrência Escherichia coli patogênica aviária (APEC) em saco vitelino de embriões, ovos bicados e saco vitelino de pintos de 1 dia. Os isolados de Escherichia coli foram submetidos à reação de PCR para identificação dos fatores preditivos mínimos de virulência, classificação filogenética e identificação preditiva dos complexos clonais ST131, ST117 e ST95. A análise fenotípica avaliou o perfil de resistência aos antimicrobianos. Foi analisado um total de 254 amostras sendo 60,63% (n=154/254) positiva para E. coli, e destas 35,71% (n=55/154) foram classificadas como APEC. Na divisão por tipo de amostra 38,82% (n=33/85) de APEC foram isolada em sacos vitelínicos de pintainhos de um dia, 29,09% (n=16/55) em ovos bicados e 42,86% (n=6/14) em ovos embrionados. Na caracterização filogenética, 36,36% (n=20/55) foram identificadas como pertencentes ao filogrupo B2. Dentre as APECs, o complexo clonal preditivo mais prevalente foi ST131(23,63%; n=13/55), seguido do ST117 (14,5%; n=8/55) e ST95 (3,6%; n=2/55). Um total de 25,45% das cepas consideradas multirresistentes (MDR), com maior índice de resistência para a amoxicilina (43,64%).
Title in English
Phenotypic and genotypic characterization of Escherichia coli isolated from chicks and embryos in the hatchery
Keywords in English
Antibiogram
APEC
Escherichia coli
Hatchery
MLST
Abstract in English
Every year, Brazil's poultry production increases, and a very important link in the production chain is the hatchery. Incubation has a significant impact on the life and quality of the chicks and is fundamental for companies to be productive and competitive in the market. However, certain diseases can compromise the viability of birds in the embryonic stage, with an impact on animal and public health. Omphalitis, the main etiological agent of which is Escherichia coli (E. coli), is a disease that affects newborn chicks up to the 7th day of life, causing high mortality and reduced performance in asymptomatic chicks. The aim of the study was to determine the frequency of occurrence of avian pathogenic Escherichia coli (APEC) in the yolk sac of embryos, pecked eggs and the yolk sac of day-old chicks. Escherichia coli isolates were subjected to PCR to identify the minimum predictive virulence factors, phylogenetic groups, and predictive identification of the clonal complex ST131, ST117 and ST95. Phenotypic analysis assessed the antimicrobial resistance profile. A total of 254 samples were analyzed and 60.63% (n=154/254) were positive for E. coli, of which 35.71% (n=55/154) were classified as APEC. In the breakdown by sample type, 38.82% (n=33/85) of APEC were isolated in the yolk sac of day-old chicks, 29.09% (n=16/55) in pecked eggs and 42.86% (n=6/14) in embryonated eggs. In the phylogenetic characterization, 36.36% (n=20/55) were identified as belonging to B2. Among the APECs, the most prevalent CC ST was ST131 (23.63%; n=13/55), followed by ST117 (14.5%; n=8/55) and ST95 (3.6%; n=2/55). A total of 25.45% were considered multidrug-resistant strains (MDR), with the highest resistance rate to amoxicillin (43.64%).
 
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Release Date
2026-07-18
Publishing Date
2024-07-23
 
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