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Master's Dissertation
DOI
10.11606/D.10.2006.tde-17042007-122417
Document
Author
Full name
Patricia Miyuki Ohara
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2006
Supervisor
Committee
Vasconcellos, Silvio Arruda (President)
Oliveira, Eugenia Márcia de Deus
Pinheiro, Sonia Regina
Title in Portuguese
Microbiologia e histopatologia de linfonodos de bovinos com lesões macroscópicas sugestivas de tuberculose. Colheitas efetuadas de Maio/2002 à Janeiro/2004, São Paulo, Brasil
Keywords in Portuguese
Bovinos
Histopatologia
Linfonodos
Microbiologia
Tuberculose
Abstract in Portuguese
Linfonodos de 146 bovinos com lesões macroscópicas identificadas como sugestivas de tuberculose pelos Serviços de Inspeção Federal e Estadual de abatedouros do Estado de São Paulo, Brasil, no período de Maio de 2002 à Janeiro de 2004, conservadas em solução de borato de sódio em temperatura ambiente por no máximo 60 dias, negativos nos cultivos de micobactérias nos meios de Lowëstein-Jensen e Stonebrink com descontaminação por Petroff e HPC e conservadas por congelamento, foram submetidos a novos cultivos em Agar Sangue, Agar MacConkey e Agar Saboraud bem como ao exame histopatológico pelas colorações de Hematoxilina-Eosina e Ziëlh-Neelsen. Nos cultivos houve isolamentos de E. faecium, E. faecalis, E. solitarius, S. arlettae e S. epidermidis. Não houve correlação entre as bactérias isoladas e a localização dos respectivos linfonodos. Os exames histopatológicos classificaram 49 animais isentos de qualquer lesão microscópica, 4 positivos para micobactérias sp, 18 fortemente sugestivos para micobactérias sp e 45 como processos atribuídos a outros agentes que não micobactérias. Não houve correlação entre o aspecto histológico dos linfonodos e o tipo de microrganismo isolado. Os microrganismos isolados foram considerados como contaminantes que tiveram acesso aos materiais durante os procedimentos de abate.
Title in English
Microbiology and histopathology of bovine lymph nodes with lesions macroscopically suggestive of tuberculosis, collected from May/2002 to January/2004, São Paulo, Brazil.
Keywords in English
Bovine
Histopathology
Lymphatic nodes
Microbiology
Tuberculosis
Abstract in English
Lymph nodes of 146 bovines presenting lesions identified macroscopically as suggestive of tuberculosis by the Federal and State Inspection Services of abbatoirs at the State of São Paulo, Brazil, from May, 2002 to January, 2004, that were preserved in sodium borate solution at room temperature for no longer than 60 days, negative to mycobacteria culturing on Lowenstein-Jensen and Stonebrink media, decontaminated by Petroff and HPC methods and kept frozen, were subjected to new cultivations on blood, MacConkey and Sabouraud agars as well as to histopathological examination by Hematoxilin-Eosin and Ziëlh-Neelsen coloration techniques. E. faecium, E. faecalis, E. solitarius, S. arlettaeand S. epidermidis were isolated by culturing. No correlation between the isolated bacteria and the respective lymph nodes localization was found. The histopathological classification of the animals was: 49 presented microscopically no lesion, 4 were Mycobacterium sp. positive, 18 were strongly suggestive of Mycobacterium sp. and 45 were attributed to agents other than mycobacteria. There was no correlation between the histological aspect of the lymphnodes and the microorganisms isolated. The isolated microorganisms were considered as contaminants that had access to these materials during the slaughter procedures.
 
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Publishing Date
2007-04-23
 
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