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Master's Dissertation
DOI
10.11606/D.10.2010.tde-29062012-135605
Document
Author
Full name
Sergio de Mello Novita Teixeira
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Moreno, Andrea Micke (President)
Calderaro, Franco Ferraro
Moretti, Anibal de Sant' Anna
Title in Portuguese
Caracterização genotípica de cepas de Pasteurella multocida proveniente de suínos
Keywords in Portuguese
Fatores de virulência
Pasteurella multocida
PCR
Suino
Toxina
Abstract in Portuguese
Um total de 123 isolados de Pasteurella multocida provenientes de suínos foi avaliado através da PCR para pesquisa de genes codificadores de capsula, toxina dermonecrótica e outros fatores de virulência. As amostras foram caracterizadas como tipo capsular A (78,8 %) e tipo capsular D (21%). Nenhum dos isolados foi positivo para presença de toxina dermonecrótica. Os genes codificadores dos fatores de virulência pesquisados foram observados nas freqüências de 93,5 % para nanB, 92,7% para psl, 91,9% para oma87 e nanH, 87,8% para sodA,87% para hghA,83,7% para ompH, 82,9% para sodC, 79,7% para ptfA e exbBD tonB,73,2% para hgbB, 14,6% para pfhA e 4,9% para tbpA. Estes achados são compatíveis com o descrito na literatura internacional.
Title in English
Genotipic characterization of Pasteurella multocida strains from swine
Keywords in English
Pasteurella multocida
PCR
Swine
Toxin
Virulence Factors
Abstract in English
A total of 123 Pasteurella multocida strains from swine was evaluated through PCR to detect capsular, dermonecrotic toxin codifying genes and others virulence factors. The strains were identified as capsular type A (78.8 %) and capsular type D (21%). None of isolates were positive to dermonecrotic toxin gene. The virulence factors genes were detected in the following frequency: 93.5 % to nanB, 92.7% to psl, 91.9% to oma87 and nanH, 87.8% to sodA, 87% to hghA, 83.7% to ompH, 82.9% to sodC, 79.7% to ptfA and exbBD tonB, 73.2% to hgbB, 14.6% to pfhA and 4.9% to tbpA. These findings were in accordance with international literature.
 
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Publishing Date
2013-05-08
 
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