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Master's Dissertation
DOI
https://doi.org/10.11606/D.11.1977.tde-20240301-152923
Document
Author
Full name
Carlos Roberto Pierobom
Institute/School/College
Date of Defense
Published
Piracicaba, 1977
Supervisor
Title in Portuguese
Indução de fitoalexinas em feijoeiro por diferentes patógenos
Keywords in Portuguese
COMPOSTOS FENÓLICOS
FEIJÃO
FITOALEXINAS
FUNGOS FITOPATOGÊNICOS
RHIZOCTONIA
Abstract in Portuguese
A produção de fitoalexinas em hipocótilos de feijoeiro infectados por Fusarium solani f. phaseoli e por Rhizoctonia solani foi estudada, em plantas da variedade “Carioca”. Duas fitoalexinas caracterizadas, uma por apresentar Rf 0,35 no sistema de Cromatografia de Camada Fina (CCF) de Hedin, fluorescer sob UV e possuir um pico de absorção de UV a 280 nm e, a outra, por apresentar Rf 0,65 no referido sistema CCF, absorver UV e possuir picos a 280 e 315 nm no espectro de absorção de UV, foram extraídas de ambos sistemas patógeno-hospedeiro. Através de CCF, absorção de UV e testes de inibição sobre fungos verificou-se que o feijoeiro produz as mesmas fitoalexinas, na mesma sequência, independentemente do patógeno utilizado como indutor.
Abstract in English
Studies of phytoalexins extracted from hypocotyls of bean plants, var. “Carioca”, infected by Rhizoctonia solani and in same tissue of this host, infected by Fusarium solani f. phaseoli, were carried out. Two phytoalexins, one with Rf 0.35 in Hedin’s Thin Layer Chromatography system, fluorescing under short UV light, with a maximum a 280 nm and, the other, with Rf 0.65 in the same TLC system, absorbing short UV light and with maxima at 280 and 315 nm, were extracted from both host-pathogen system. Using TLC, UV spectrum and effect on mycelial growth and spore germination of fungi, was verified that the bean plant produces the same phytoalexins, in the same sequence not depending on the host-pathogen system.
 
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Publishing Date
2024-03-14
 
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