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Master's Dissertation
DOI
https://doi.org/10.11606/D.11.1986.tde-20220207-205845
Document
Author
Full name
Jose Antonio Martinelli
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 1986
Supervisor
Title in Portuguese
Meios de cultura e condições ambientes favoráveis ao crescimento e esporulação in vitro de Microcylus ulei (P. Henn.) v. Arx., agente do mal das folhas da seringueira (Hevea brasiliensis) (Muell.) Arg
Keywords in Portuguese
CONDIÇÕES AMBIENTAIS
CRESCIMENTO
ESPORULAÇÃO IN VITRO
FUNGOS FITOPATOGÊNICOS
MAL-DAS-FOLHAS
MEIOS DE CULTURA
SERINGUEIRA
Abstract in Portuguese
O presente trabalho versa sobre a obtenção de um meio de cultura equilibrado para proporcionar abundante esporulação e crescimento da fase imperfeita de Microcylus ulei (P. Henn.) v. Arx, Fusicladium macroposrum Kuyper. Após a elaboraçio de um meio sintético basal determinou-se a melhor fonte de carbono, a melhor fonte de nitrogênio e a melhor relação C/N para as fontes testadas e a influência de requisitos nutricionais mínimos tais como vitaminas e aminoácidos. No estudo visando obter abundante esporulação de M. ulei, verificou-se que a utilização de galactose e soitone, sobre um meio basal sintético, como fontes de carbono e de nitrogênio, respectivamente, ambas a 10,52 g/litro, mantendo uma relação C/N de 1:1 e incubadas a 23°C sob períodos de luz alternados a cada 24 horas, durante 90 minutos, no estádio final de desenvolvimento da cultura, foram as condições que apresentaram os melhores resultados.
Title in English
Culture media ano favourable environmental conditions to growth and esporulation in vitro of Microcylus ulei (P. Henn.) v. Arx., causative agent of south american leaf blight of hevea rubber trees (Hevea brasiliensis) (Muell.) Arg
Abstract in English
This work treats. to obtain an equilibrated culture medium to give high sporulation of the imperfect stage of Microcylus ulei (P. Henn.) v. Arx, Fusicladium macroposrum Kuyper. After the e laboration of a basal syntetic medium, it was determined the best source of carbon, the best source of nitrogen, the best C/N relationship . and the influence of the minimum nutritional requirements as vitamins and aminoacids. Temperatures, brightness and incubation time was studied too. With the studies in order to get high sporulation of M. ulei, have been seen the utilization of galactose and soytone, in a sintetic basal medium, as sources of carbon and nitrogen, respectively, both at 10,5 2 g/l , with a relationship C/N of 1:1 and incubated at 23°C under alternated periods of light each 24 hours during 90 minutes, at the final stage of development of the culture,were the conditions that showed the best results.
 
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Publishing Date
2022-02-07
 
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