• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Doctoral Thesis
DOI
https://doi.org/10.11606/T.11.1993.tde-20231122-093359
Document
Author
Full name
Antonio Carlos Maringoni
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 1993
Supervisor
Title in Portuguese
Detecção de Xanthomonas campestres PV. phaseoli (SMITH) dye em sementes de feijoeiro e consequências epidemiológicas
Keywords in Portuguese
BACTÉRIAS FITOPATOGÊNICAS
CRESTAMENTO BACTERIANO COMUM
EPIDEMIOLOGIA
FEIJÃO
SEMENTES
SEROLOGIA
Abstract in Portuguese
Foi constatada a variabilidade sorológica entre isolados de X. campestres pv. phaseoli através da técnica de dupla difusão em gel-de-ágar. Antígenos de X. campestres pv. phaseoli e da variante fuscans reagiram apenas com os antissoros homólogos; entre os isolados da variante fuscans houve dois que não com o antissoro homólogo. O meio de cultura semi-seletivo desenvolvido, constituído de : extrato de carne - 3,0 g, peptona - 5,0 g, sacarose - 10 g, amido solúvel 2, 0 g, ágar - 15,0 g, água destilada - 1000 ml, acrescido, após a autoclavagem, a 45 - 50 °C de chlorothalonil - 20 μg/ml, benomyl - 20 μg/ml, cefalexina - 30 μg/ml, ácido nalidixico - 1 μg/ml e nitrofurantoina - 2 μg/ml, foi eficiente no isolamento de X. campestres pv. phaseoli de sementes de feijão naturalmente infectadas. Verificou-se que o processo de extração da bactéria das sementes através de trituração propiciou uma maior recuperação da X. campestris pv. phaseoli quando comparado com a maceração. Nos ensaios conduzidos em campo, nas safras das águas de 1988 e 1989 e da seca de 1990, evidenciou-se maior nível de resistência horizontal à X. campestres pv. phaseoli na variedade IAPAR 16, tanto nos folíolos quanto na vagem. As variedades Carioca e IAPAR 20 foram mais suscetíveis a essa bacteriose e a variedade IAPAR 20 apresentou menor produção e peso de mil sementes. O sistema de avaliação do crestamento bacteriano comum através da severidade (porcentagem de área foliar infectada por folíolo) foi mais eficiente do que a incidência (porcentagem de folíolos doentes) para quantificar a doença nos folíolos e permitiu discriminar os diferentes níveis de resistência horizontal presentes nas variedades de feijoeiro ensaiadas. O desenvolvimento da epidemia do crestamento bacteriano comum nas variedades de feijoeiro ensaiadas dependeu mais do nível de resistência horizontal presente nas variedades e das condições climáticas favoráveis do que da população de X. campestres pv. phaseoli presente nas sementes.
Title in English
Detection of Xanthomonas campestres pv. (SMITH) dye on dry bean seeds and epidemiological consequences
Abstract in English
The serological variability among X. campestris pv. phaseoli strains was detected through the agar double diffusion method. Antigens of X. campestris pv. phaseoli and fuscans strains reacted only with the homologous antisera. There were two fuscans strains that did not react whith the homologous antiserum. The semiselective medium developed (beef extract - 3,0 g, peptone - 5,0 g, sucrose - 10,0 g, soluble starch - 2,0 g, agar - 15, 0 g, distilled water - 1000 ml, plus chlorothalonil - 20 μg/ml, benomyl - 20 μg/ml, cephalexin - 30 μg/ml, nalidixic acid - 1 μg/ml and nitrofurantoin - 2 μg/ml, added after sterilization at 45-50°C) was efficient to recover X. campestris pv. phaseoli from naturally infected bean seeds. The extraction of this bacterium from seeds by crunching was more efficient than by maceration. The experiments carried out in the field during wet (1988 and 1989) and dry (1990) seasons showed the greatest level of horizontal resistance of leaves and pods of bean cultivar IAPAR 16 to X. campestris pv. phaseoli. Bean cultivars Carioca and IAPAR 20 were more susceptible than IAPAR 16 to the disease. IAPAR 20 however had lower yield and weight of thousand seeds than Carioca and IAPAR 16. Disease evaluation by a severity index (percentage of diseased area per leaflet) was better than incidence (percentage of diseased leaflet) for discriminating the levels of horizontal resistance to bean common bacterial blight among bean cultivars. The development of the common bacterial blight epidemic in IAPAR 16, Carioca and IAPAR 20 cultivars depended more on the level of horizontal resistance and climatic conditions than on the X. campestris pv. phaseoli populations present in bean seeds.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2023-11-24
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
CeTI-SC/STI
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2024. All rights reserved.