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Master's Dissertation
DOI
10.11606/D.11.2013.tde-13112013-151232
Document
Author
Full name
Ivan Miletovic Mozol
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 2013
Supervisor
Committee
Labate, Carlos Alberto (President)
González, Esteban Roberto
Vitorello, Claudia Barros Monteiro
Title in Portuguese
Análise temporal da expressão gênica e atividade enzimática, relacionadas ao estresse oxidativo e proteômica de Eucalyptus grandis inoculados com Puccinia psidii
Keywords in Portuguese
Eucalipto
Ferrugem
PCR em tempo real
Proteoma
Abstract in Portuguese
A floresta plantada de eucalipto representa a maior porcentagem entre as florestas plantadas no Brasil, principalmente para a producao de papel e celulose. Porem, durante todo o seu desenvolvimento, o eucalipto sofre o ataque de diferentes patogenos, e entre eles esta o fungo Puccinia psidii causador da ferrugem, principal doenca do eucalipto em regioes tropicais. Assim, com o intuito de estudar alguns mecanismos de defesa da planta contra a infeccao do fungo, este trabalho visou analisar a expressao genica de enzimas relacionadas ao estresse oxidativo e a atividade das mesmas; e o proteoma de clones de eucalipto resistentes e suscetiveis, ao longo do processo de infeccao, colonizacao e multiplicacao do fungo nas plantas. Foi possivel observar diferencas na expressao dos genes em todos os horarios estudados, principalmente as 24 horas apos inoculacao com o fungo. Alem disso, com a analise do proteoma pode-se observar algumas proteinas de grande relevancia para este trabalho, como algumas relacionadas a estresse oxidativo e a resposta de defesa da planta.
Title in English
Temporal analysis of gene expression and enzyme activity related to the oxidative stress, and proteomics of Eucalyptus grandis inoculated with Puccinia psidii
Keywords in English
Eucalyptus
Proteome
Real Time PCR
Rust
Abstract in English
The eucalyptus planted forest represents the major percentage among all planted forests in Brazil, mainly for the production of paper and cellulose. However, during its development, the eucalyptus can be attacked by a large number of different pathogens, like the fungus Puccinia psidii, the causer of the eucalyptus rust, main disease that affects the eucalyptus in tropical regions. Thus, in order to study some plant defense mechanisms against the infection of the fungus, this study aimed to analyse the gene expression of some enzymes related to oxidative stress and their activities, and the proteome of resistant and susceptible eucalyptus clones, during the process of infection, colonization and multiplication of the fungus in the plant. We could observe differences in the gene expression at all times studied, mostly at 24 hours after inoculation with the fungus. Besides, with the proteome analysis we could find the very relevant proteins for this study, for instance some proteins related to the oxidative stress and to the plant defense response.
 
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Publishing Date
2013-11-19
 
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