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Master's Dissertation
DOI
Document
Author
Full name
Alexandre Oliveira de Souza
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 1996
Supervisor
Title in Portuguese
Bactérias endofíticas de milho (Zea mays L.) e sua variabilidade genética analisada por RAPD
Keywords in Portuguese
BACTÉRIAS ENDOFÍTICAS
MARCADOR MOLECULAR
MILHO
VARIAÇÃO GENÉTICA
Abstract in Portuguese
Foram isoladas 169 colônias de bactérias endofíticas de folhas de diferentes genótipos de plantas de milho sadias, pertencentes às populações BR-105, BR-106 e população de híbridos simples entre essas, em duas localidades e coletas distintas. Trinta isolados foram identificados por não de testes morfológicos e bioquímicos clássicos, definindo-se a ocorrência e porcentagem relativa dos gêneros: Bacillus (55%), Corynebacterium (15%), Micrococcus (12%), Listeria (9%), Pseudomonas (6%) e Erwinia (3%). A técnica de RAPD foi aplicada a treze isolados do gênero Bacillus, o mais ocorrente, para medir o grau de similaridade genética entre esses e linhagens de referência, não endofíticas de Bacillus megaterium e Bacillus subtilis, e relacionar a origem dos isolados com o local de coleta e número da coleta. A análise de RAPD permitiu a diferenciação de quatro grupos entre Bacillus sp. endofíticos e as linhagens de referência, com aproximadamente de 32% de similaridade entre si. Revelou-se grande variabilidade dentro do grupo, havendo relação dentro de dois grupos com o local de coleta e o número de coleta. Foi realizado um ensaio in vitro de antagonismo fungo-bactéria endofítica, entre dez isolados de bactérias, sendo os principais gêneros testados, Bacillus e Micrococcus e os fungos endofíticos Penicillium purpurogenum, P. spinulosum e Fusarium moniliforme, todos isolados de milho, e duas linhagens produtoras de antibióticos não endofíticas de P. chrysogenum IFO-4626 e IZ1671. Este ensaio revelou não haver inibição do crescimento das bactérias pelos fungos endofíticos. Entretanto dois isolados endofíticos foram inibidos pelas linhagens de P. chrysogenum, indicando um tipo de associação diferente da que ocorreu entre os endofíticos, caracterizada por uma antibiose
Title in English
Maize (Zea mays L.) endophytic bacteria and their genetic variability analysis by RAPD
Abstract in English
Endophytic bacteria were isolated in healthy maize leaves from different genotypes of BR-105 and BR-106 populations and from their simple hybrids population. Isolation was performed on two experimental areas by two distinct harvests. The total number of isolated bacteria colonies was 169. Thirty isolates were identified by classic morphologic and biochemical tests, and defined the genus occurence and relative percentage: Bacillus (55%), Corynebacterium (15%), Micrococcus (12%), Listeria (9%), Pseudomonas (6%) and Erwinia (3%). RAPD technique was performed to measure the genetic similarities among the thirteen main occurring Bacillus endophytes, and the reference strains not endophytes Bacillus megaterium and Bacillus subtilis, also relating the endophyte origins with the harvest area and harvest number. RAPD analysis allowed to differentiate four groups among Bacillus endophytes and Bacillus reference strains, presenting nearly 32% of similarity themselves. High variability was detected within the group, being detected within two groups relationships with harvest area and harvest number. It was performed an in vitro endophyte bacteria- fungi antagonism assay, with ten bacteria isolates, being the major genus assayed Bacillus and Micrococcus, and the endophytes Penicillium purpurogenum, P. spinulosum and Fusarium moniliforme, all of them isolated from maize, and two antibiotic producers, not endophytes, P. chrysogenum IF04626 and IZ1671. Such assay has shown that endophytic fungi was not able to inhibit endophytic bacteria growth. Althoug, two endophytes were inhibited by antibiotic producers lineages P. chrysogenum, indicating a different kind of association from that occuring among endophytes, characterized by antibiosis
 
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Publishing Date
2019-08-22
 
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