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Mémoire de Maîtrise
DOI
https://doi.org/10.11606/D.11.1985.tde-20210919-110123
Document
Auteur
Nom complet
Teresa Losada Valle
Unité de l'USP
Domain de Connaissance
Date de Soutenance
Editeur
Piracicaba, 1984
Directeur
Titre en portugais
Influência da qualidade da luz no crescimento e esporulação do Metarhizium anisopliae (Metsch,) Sorokinn
Mots-clés en portugais
CRESCIMENTO
ESPORULAÇÃO
FUNGOS ENTOMOPATOGÊNICOS
QUALIDADE DA LUZ
Resumé en portugais
O presente trabalho verificou a influência da qualidade da luz na esporulação e crescimento radial do fungo entomopatogênico Metarhizium anisopliae (Metsch.) Sorokin. Para isso utilizaram-se seis linhagens: A4, A19, E9, K, M e P; três meios de cultura: Meio Completo (MC), Meio de Arroz (MA) e Batata-dextrose ágar (BDA); seis tratamentos com luz: ?BL? (?black light?), azul, verde, amarelo, vermelho e dia, e uma testemunha (escuro). Verificou-se que o crescimento radial foi mais influenciado pelo meio de cultura do que pela luz. A produção total de conídios aumentou, em média 116%, quando as linhagens foram cultivadas sob luz, mas a amplitude da resposta foi função do meio de cultura, da linhagem e da qualidade de da luz. Linhagens com baixa produção de conídios em MC e BDA recuperaram a capacidade de esporulação em MA mesmo sem tratamento luminoso. A viabilidade dos conídios diminuiu significativamente na linhagem E9, quando cultivado em MC e iluminada com luzes azul, verde, vermelho, amarelo ou dia.
Titre en anglais
Influence of light quality in growth and sporulation in Metarhizium anisopliae (Metsch,) Sorokin
Resumé en anglais
The present work was carried out in order to study the influence of the quality of light in the radial growth and sporulation of the entomopathogenic fungus Metarhizium anisopliae (Metsch.) Sorokin. It was used six fungus strains, three growth media (complete medium - CM, rice medium - RM and potato-dextrose-agar - PDA). The types of light used were: ?black-light? (BL), blue, green, yellow, red and day lights, and also a check treatment (dark). The radial growth was less influenced by the qualities of light that by the growth media. The best medium was the CM; PDA was superior to the RM. The total yield of conidia increased, in average, 116% when the strains were under light, in relation to dark. The magnitude of response, however was function of the growth medium, the strain, and the quality of light. Strains with low conidia production, where grown on CM and PDA, recovered their capacity of sporulation in RM, even in the dark. The strain E9 had its conidia viability reduced significantly when grown on MC, under blue, green, red, yellow or day light. Every quality of light increase significantly the production of conidia, but for a maximum yield, it is necessary to determinate the best light, in relation to each strain and growth medium.
 
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ValleTeresaLosada.pdf (7.75 Mbytes)
Date de Publication
2021-09-19
 
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