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Master's Dissertation
DOI
10.11606/D.11.2018.tde-20181127-155151
Document
Author
Full name
Célia Regina Bertechini Gomes Duque
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 1991
Supervisor
Title in Portuguese
Efeito da temperatura de ativação, ph e tempo de incubação na germinação de esporos de bactérias isoladas de processos de fermentação alcoólica
Keywords in Portuguese
BACTÉRIAS
FERMENTAÇÃO ALCOÓLICA
GERMINAÇÃO DE ESPOROS
INCUBAÇÃO
PH
TEMPERATURA
Abstract in Portuguese
O presente trabalho visou basicamente estudar a influência dos fatores ph, temperatura de ativação e tempo de incubação na germinação dos esporos de bactérias isoladas da fermentação alcoólica. Para ativação, os esporos de Bacillus subtilis, Bacillus coagulans, e Bacillus megaterium foram submetidos aos aquecimentos a 60, 80 e 90 ºC em caldo de cana-de-açúcar a ph 4,5; 5,5 e 6,5. Depois do tratamento de ativação, as suspensões de esporos foram resfriadas à aproximadamente 35ºC, e em seguida, pré-incubadas a 35,0 ± 1,0 ºC por 6 horas. Amostras foram coletadas a 0, 2, 4 e 6 horas, diluídas serialmente, plaquadas e incubadas a 35,0 ± 1,0 ºC por 24 horas. O número de colônias foi tomado como o número de esporos ativados e germinados. A análise estatística dos dados permite concluir que houve uma diminuição no número de esporos germinados, para todas as espécies de Bacillus empregadas, com o aumento da temperatura e tempo de incubação, sendo que a ativação a 90 ºC provocou uma certa inativação dos mesmos. Houve também redução no número de esporos germinados para todos os níveis de ph, com o aumento do tempo de incubação, e o ph 4,5 mostrou um efeito inibitório mais acentuado na germinação dos esporos, exceto para Bacillus brevis. De uma maneira geral, verificou-se que o melhor tratamento para a ativação e germinação dos esporos das quatro espécies investigadas, em meio de caldo de cana-de-açúcar foi 80 ºC e ph 5,5.
Title in English
Effect of pH, activation temperature and incubation time on germination of bacteria spores isolated from alcoholic fermentation process
Abstract in English
This study was undertaken to investigate the influence of pH, activation temperature and incubation time on germination of bacteria spores isolated from alcohol fermentation process. The spores of Bacillus subtilis, Bacillus coagulans, Bacillus brevis and Bacillus megaterium were activated by heating them at 60, 80 and 90 ºC in sugar- cane- juice at pH 4,5, 5,5 and 6,5. After the activation treatments the spores suspensions were cooled to 35,0 ºC and then pre-incubated at 35,0 ± 1,0 ºC for 6 hours. Samples were withdrawn at 0, 2, 4 and 6 hours, serial diluited plated and incubated at 35,0 ± 1,0 ºC for 24 hours. The number of colonies developed was taken as the number of spores activated and germinated. The statiscal analysis of data showed that there was a reduction of the number of spores germinated for all four species of Bacillus, as the temperature of activation and time of incubation increased. The higher activation temperature (90 ºC) showed and inactivation effect on the spores. There was also a reduction of the spores germinated for all pH levels with the increase of the incubation time, and pH 4,5 showed a higher inhibitory effect on the spore germination, with exception for B. brevis spores. In the overall, the best treatments for the spores activation and germination in sugar-cane-juice, was 80 ºC and pH 5,5, what means that they may germinated if present, in the sugar-cane-juice, at the heat treatment conditions, for a sugar and alcohol production.
 
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Publishing Date
2018-11-27
 
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