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Master's Dissertation
Full name
Aparecido Osdimir Bertolin
Knowledge Area
Date of Defense
Piracicaba, 1987
Title in Portuguese
Isolamento e biologia de novos fungos celulolíticos
Keywords in Portuguese
Abstract in Portuguese
Para viabilizar o aproveitamento da celulose, e necessário sua hidrólise obtendo-se resíduos de glicose; esta hidrólise pode ser ácida ou enzimática. A hidrólise enzimática apresenta algumas vantagens sobre a ácida, embora microrganismos utilizados até agora não sejam extremamente eficientes na produção de celulases. Com o interesse de explorar melhor a nossa microbiota, o presente trabalho se propôs a busca de fungos com alta capacidade de hidrolisar celulose, isolados a partir de duas regiões geográficas diferentes. Foram assim obtidos 20 isolados que foram analisados e comparados com um padrão o Trichoderma reesei QM 9414 considerado como alto produtor de celulases. Foi feito um estudo de aspectos biológicos dos 20 isolados, fixando assim bons parâmetros para estudos posteriores no tocante a classificação e melhoramento genético destes isolados. Especialmente um dos isolados classificado como Trichoderma pseudo conjngii foi considerado promissor por apresentar uma alta taxa de produção de celulases além de apresentar outras características favoráveis para futuros estudos de genética e melhoramento.
Title in English
Isolation and biology of new cellulolytic fungi
Abstract in English
For better uses for cellulose it must be partetioned in to glucose residues and this can be done by the acid or by enzymatic hydrolysis. Some advantages can be pointed out in using the enzymatic process although strains of microorganisms used until now can still be improved to produce higher amounts of cellulases. The main goal of this work was to explore our microbiota aiming to find out new fungi with high ability in breaking down 'cellulose. Tow different geographic regions were select and from them 20 isolateds were obtained and tested. Trichoderma reesei QM 9414 was used a standard for comparing the performance of the isolated fungi strains. Morphological and biological parameters were stablished for the 20 strains providing informations for future classification and genetic improvement programs. One of the isolates classified as Trichoderma pseudo coningii, was shown to be the most promessing one giving high rates of cellulases production; it also presented favorable characteristics for genetic and breeding studies.
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