• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Master's Dissertation
DOI
https://doi.org/10.11606/D.11.1983.tde-20231122-100616
Document
Author
Full name
Ofélia Cleusa Rosante Gomes
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 1982
Supervisor
Title in Portuguese
Resistência a metais pesados e defensivos agrícolas em Metarhizium anisopliae (Metsch.) Sorokin
Keywords in Portuguese
FUNGOS ENTOMOPATOGÊNICOS
METAIS PESADOS DO SOLO
PESTICIDAS
RESISTÊNCIA
Abstract in Portuguese
O objetivo do presente trabalho foi estudar a resistência da linhagem E6 de M. anisopliae (Metsch) Sorokin, à alguns sais minerais [MnSO4, HgCl2, CuSO4, ZnSO4 e Pb (CH3COO)2] e defensivos químicos (Malathion, Decamethrina, Fenvalerate, Dimethoate e Bihedonal), em diferentes concentrações. Medidas de crescimento (diâmetro das colônias) e de germinação (número de colônias/placa) foram feitas com a finalidade de verificar a inibição pelas substâncias usadas. Os resultados obtidos indicam que, dentre os sais, o HgCl2 foi o mais tóxico ao fungo, seguindo-se pela ordem de inibição, CuSO4, Pb (CH3COO)2, MnSO4 e ZnSO4. Em alguns sais (MnSO4, ZnSO4 e CuSO4) apareceram setores que foram ensaiados em concentrações bem altas dos referidos sais, a fim de se verificar se eram variantes resistentes a dosagens mais altas. O variante isolado do meio de cultura com CuSO4, apresentou o mesmo comportamento que a linhagem original em relação ao efeito do sal. Os variantes isolados do MnSO4 e ZnSO4 mostraram alta resistência ao respectivo sal. Foram observadas diferenças morfológicas que juntamente com outros processos, poderão ser utilizados para caracterização do fungo. Quanto aos defensivos, todos inibiram o fungo, sendo que destes, o mais tóxico foi Decamethrina, que inibiu completamente o crescimento na concentração de 40 µg/ml, seguindo-se, pela ordem de inibição: Fenvalerate, Malathion, Dimethoate e Bihedonal.
Title in English
Not available
Abstract in English
This research was undertaken to study the resistance of the strain E6 of Metarhizium anisopliae (Metsch) Sorokin, to some mineral salts [MnSO4, HgCl2, CuSO4, ZnSO4 and Pb (CH3COO)2] and chemical defenses (Malathion, Decamethrina, Fenvalerate, Dimethoate and Bihedonal) in different concentrations. Growth and germination measurements (colony diameters and number of colonies/dish) were made in order to verify inhibition caused by the elements used. The results obtained indicate that, among the salts, HgCl2 was the most toxic for the fungus, being followed by - in order of inhibition - CuSO4, Pb (CH3COO)2, MnSO4 e ZnSO4. Sectors appeared in some of the salts (MnSO4, CuSO4 e ZnSO4) which were tested in highly concentrated doses in order to verify if they were more resistant variants to large doses of the respective salts. The variant isolated from the culture medium with CuSO4, demonstrated the same behavior as the original strain in relation to the effects of the salt. The isolated variants of MnSO4 e ZnSO4 demonstrated high resistance to the respective salt. Morphologic differences were observed, which, together with other processes, can be utilized for the characterization of the fungus. As regards the defensives used, all of them inhibited fungus growth and sporulation. Among these defensives, Decamethrina was the most toxic, inhibiting growth in the concentration of 40 µg/ml, being followed, in order of inhibition, by Fenvalerate, Malathion, Dimethoate e Bihedonal.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2023-11-24
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
CeTI-SC/STI
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2024. All rights reserved.