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Master's Dissertation
DOI
https://doi.org/10.11606/D.11.2018.tde-20181127-160526
Document
Author
Full name
Norimar D'avila Denardin
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 1991
Supervisor
Title in Portuguese
Seleção de estirpes de Rhizobium leguminosarum bv, phaseoli tolerantes a fatores de acidez e resistentes a antibióticos
Abstract in Portuguese
De 31 amostras de solo, coletadas em regiões de cultivo de feijoeiro (Phaseolus vugaris), no estado de São Paulo foram obtidos 151 isolados de Rhizobium leguminosarum bv. phaseoli. Esses rhizobios, isolados a partir de nódulos radiculares em plantas de feijoeiro, foram purificados, autenticados, descritos morfologicamente e submetidos a uma seleção, quanto a eficiência simbiótica, tolerância a fatores de acidez, pH 4,5 e pH 4,5 + 100 uM, de alumínio, e resistência a antibióticos. Parte desses isolados foram testados para inoculação cruzada em plantas de Leucaena leucocephala, objetivando-se avaliar a habilidade infectiva e a eficiência simbiótica nesta leguminosa. Os isolados apresentaram elevada variabilidade comportamental quanto a eficiência simbiótica, tolerância à acidez e resistência aos 7 níveis dos 7 antibióticos estudados. Os resultados permitiram destacar estirpes com atividades da nitrogenase superiores às da testemunha CM 02 (CIAT 899), tolerância aos fatores de acidez e resistência aos antibióticos. De 140 isolados testados para infectar e fixar nitrogênio em Leucaena leucocephala, 106 formaram nódulos, sendo que 99 destes apresentaram eficiência simbiótica e 57 toleraram aos fatores de acidez
Title in English
Selection of strains of Rhizobum leguminosarum bv. phaseoli tolerant to acidity factors and resistant to antibiotics
Abstract in English
From 31 soil samples used, 151 strains of Rhizobium leguminosarum bv. phaseoli were isolated. These strains were isolated from nodules obtained from commom bean (Phaseolus vulgaris) plant purified, identified morphologically described and selected for efficiency in N2 fixation, tolerance to acidity factors (pH 4.5 and pH 4.5 + 100 uM A1) and resistance to antibiotics. To evaluate cross-inoculation ability strains were inoculated in Leucaena leucocephala plants. The isolates showed high variability in symbiotic efficiency for N2- fixation, tolerance to soil acidity factors and resistance to antibiotics studied. Strains with nitrogenase activity higher than the control, tolerant to acidity factors and resistant to antibiotics were selected. From 140 isolates tested for infection and nitrogen fixation in Leucaena leucophala plant 106 nodulated, and from these 99 showed symbiotic effectiveness and 57 tolerance to acidity factors
 
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Publishing Date
2018-11-27
 
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