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Master's Dissertation
DOI
https://doi.org/10.11606/D.11.2019.tde-20191218-160326
Document
Author
Full name
Agnaldo Scarassati
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, None
Supervisor
Title in Portuguese
Análise do desenvolvimento de gemas adventícias de Pinus Elliottii Engelm. var. elliottii in vitro
Keywords in Portuguese
DESENVOLVIMENTO IN VITRO
GEMAS
PINHEIRO
PLÂNTULAS
Abstract in Portuguese
Explantes cotiledonares e epicotileodnares de plântulas de Pinus Elliottii var. elliotii, foram cultivados em três diferentes meios de cultura, Greesshoff e Doy (1972), Murashige e Skoog (1962) e Eeuwens (1976), com duas consistências, gel e líquida e submetidos a diversas concentrações de BAP para avaliação do crescimento e desenvolvimento. A análise dos resultados permitiu concluir que para o crescimento e desenvolvimento de explantes cotiledonares, em Taxa de Crescimento Relativo (TCR), os meios de cultura mais adequados foram o Murashige e Skoog (1962) modificado (MS), Eewens (1976) modificado (EE) e ZX, na ausência de regulador de crescimento; em comprimento Murashige e Skoog (1962) modificado (MS), ZX e Gresshoff e Doy (1972) modificado (GD) sem regulador de crescimento e consistência líquida; número de gemas, Eeuwens (1976) modificado (EE) e Murashige e Skoog (1962) modificado (MS) e consistência gel; número de gemas maiores que 5 mm, ZX e consistência gel com 0,5 ou 1,0 mg/l de BAP. Outra conclusão é que para o crescimento e desenvolvimento de explantes epicotiledonares, em TCR (Taxa de Crescimento Relativo) o meio de cultura mais adequado foi o ZX, na consistência líquida, e com 2,5 mg/l de BAP; em número de gemas, Eeuwens (1976) modificado (EE) e Murashige e Skoog (1962) modificado (MS); para número de gemas maiores que 5 mm, ZX e consistência líquida com 2,5 ou 5,0 mg/l de BAP
Title in English
Shoot growth and development of Pinus elliottii Engelm. var. elliottii in vitro
Abstract in English
The aim of this research was to evaluate the growth and development of cotyledonary and epicotyledonary explants of Pinus elliottii var. elliottii cultivated in three culture different media, Gresshoff & Doy (1972), Murashige & Skoog (1962) and Eeuwens (1976), liquid and gel media supplemented with different BAP concentrations. The analysis of the results allowed to draw the following conclusions: 1 - The relative growth rates of the cotyledonary explants were higher in Murashige & Skoog (1962) modified (MS), Eeuwens (1976) modified (EE) and ZX devoid of BAP; 2 - The length growths of the cotyledonary explants were higher in liquid MS modified, ZX and Gresshoff & Doy (1972) modified (GD) devoid of BAP; 3 - The number of induced shoots were higher in solid Murashige & Skoog (1962) modified (MS) and Eeuwens (1976) modified (EE) with 0.5 or 1.0 mg/l of BAP; 4 - The number of shoots higher than 5 mm were larger in solid ZX with 0.5 or 1.0 mg/l of BAP; 5 - The relative growth rates of the epicotyledonary explants were higher in liquid ZX with 2,5 mg/l of BAP; 6 - The number of induced shoots were higher in liquid and solid Eeuwens (1976) modified (EE) and Murashige & Skoog (1962) modified (MS) with 2.5 or 5.0 mg/l of BAP. 7 - The number of shoots higher than 5 mm were larger in liquid ZX with 0.5 or 1.0 mg/l of BAP.
 
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ScarassatiAgnaldo.pdf (8.42 Mbytes)
Publishing Date
2019-12-19
 
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