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Master's Dissertation
DOI
https://doi.org/10.11606/D.11.2019.tde-20191218-161853
Document
Author
Full name
Roberto Wagner Cavalcanti Raposo
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 1989
Supervisor
Title in Portuguese
Inoculação de fungos micorrízicos vesículo-arbusculares e Bradyrhizobium spp. em caupi (Vigna unguiculata (L.) Walp.)
Keywords in Portuguese
BRADYRHIZOBIUM
CAUPI
FIXAÇÃO BIOLÓGICA DE NITROGÊNIO
FUNGOS MICORRÍZICOS
INOCULAÇÃO
SIMBIOSE
Abstract in Portuguese
Para determinar o fungo micorrízico vesículo-arbusculares que possibilite uma melhor interação na tríplice simbiose fungo MVA-caupi-Rhizobium e, selecionar as melhores estirpes de Bradyrhizobium spp. para caupi, na nodulação e fixação biológica de nitrogênio, dois experimentos foram instalados, em casa-de-vegetação, utilizando-se dois cultivares de caupi: BR-08 Caldeirão e IPEAN V- 69. No primeiro experimento, um solo tipo areia quartzosa de Belém (PA), da Amazônia, com 23 ppm de P e 68% de saturação em bases, após a calagem, foi colocado em vasos de barro com 2,5 kg de capacidade. O delineamento foi um fatorial inteiramente casualizado com quatro tratamentos (Fungos Nativos, Acaulospora morrowae, Glomus etunicatum e Gigaspora margarita) e duas cultivares já mencionadas acima, com quatro repetições. O solo dos três tratamentos foi esterilizado com brometo de metila. Todos os tratamentos foram inoculados com Bradynhizobium sp., estirpe C-516. As plantas foram colhidas para análise aos 81 dias apos a germinação. No segundo experimento, desenvolvido em vasos de Leonard modificado, sete estirpes de Bradynhizobium sp. (CM 1525, CM 1527, CM 1528, C-516, SMS 84, SMS 85 e SEMIA 6145), foram testadas contra dois controles (com 100 ppm de N e sem N), com quatro repetições. Aos quarentas e oito dias apos a emergência as plantas foram colhidas para análise. Houve um maior desenvolvimento vegetal e acúmulo de nutrientes nas duas cultivares quando foram introduzidos os fungos micorrízicos, tendo ocorrido diferenças a nível de cultivar sendo A. morrowae ou G. etunicatum os mais eficientes para BR-08 Caldeirão e, G. margarita ou G. etunicatum para o cultivar IPEAN V-69. As estirpes SEMIA 6145, CM 1527 e CM 1528 foram as melhores para os dois cultivares em todos os parâmetros estudados. Notou-se certo grau de especificidade hospedeira para o isolado CM 1525, o qual foi eficiente para o cultivar IPEAN V-69 e não para o cultivar BR-08 Caldeirão.
Title in English
Inoculation of vesicular-arbuscular mycorrhizal fungi and Bradyrhizobium spp. in cowpea (Vigna unguiculata (L.) Walp.)
Abstract in English
In order to determine the effect of vesicular-arbuscular mycorrhizal fungi in the tripartite symbiosis VAM-cowpea-Rhizobium, and to select the best strains of Bradyrhizobium spp. for cowpea, two experiments were carried out in greenhouse using two cultivars (BR-08 Caldeirão and IPEAN V-69). In the first experiment, a quartz sand soil from Belém (PA) - Amazon, with 23 ppm P and 68% of basis saturation, after liming, was put in clay pots with 2.5 kg of capacity. The fatorial design consisted of four treatments (Native fungi, Acaulospona morrowae, Glomus etunicatum and Gigaspora margarita) and two cowpea cultivars (mentioned above), with four aplications. The soil was sterilized using methyl bromide. All the treatments were inoculated with Bradyrhizobium sp., strain C-516. Plants were harvested 81 after germination. In the second experiment, carried out in Leonard jars, seven strains of Bradyrhizobium sp. (CM 1525, CM 1527, CM 1528, C-516, SMS 84, SMS 85 and SEMIA 6145), were tested against two controls (with 100 ppm of N and without N), with four replications. After 48 days, the plants harvest. There was a greater development and nutrient uptake for both cultivars when the mycorrhizal fungi were inoculated. It was found differences on the effect of VAM fungus species on cowpea development, being A. morrowae or G. etunicatum the best species for the cultivar BR-08 Caldeirão and G. margarita or G. etunicatum for the cultivar IPEAN V-69. The strains SEMIA 6145, CM 1527 and CM 1528 were the best for cultivars BR-08 Caldeirão and IPEAN V-69 in all parameters studied. However, there was a certain degree of specificity with the strain CM 1525 being efficient for cultivar IPEAN V-69 and not for cultivar BR-08 Caldeirão.
 
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Publishing Date
2019-12-19
 
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