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Master's Dissertation
Full name
Luis Roberto Martin
Knowledge Area
Date of Defense
Piracicaba, 1993
Title in Portuguese
Isolamento e identificação por taxonomia numérica da microbiota lática do leite cru
Keywords in Portuguese
Abstract in Portuguese
Para o levantamento da microbiota bacteriana do leite cru, amostras de leite foram coletadas durante o período de Maio de 1990 a Janeiro de 1991, junto a Indústria de Laticínios Colina Ltda, distrito de Ártemis, Piracicaba - SP. A partir de placas em APT-S e meio seletivo para Leuconostoc foram selecionadas 156 culturas para serem identificadas através de matrizes de similaridade colocadas em um programa de computação. O gênero Lactococcus se constituiu no microrganismo mais frequente da microbiota lática (42,31%), seguido de Pediococcus (28,20%), Leuconostoc (17,31%), Lactobacillus (10,26%) e Streptococcus (1,92%). Os isolados de Lactococcus foram classificados como L. raffinolactis (22,44%) e L. lactis subsp. lactis (19,87%). A classificação de Pediococcus apresentou P. inopinatus (28,21%). Os isolados de Leuconostoc foram classificados como L. lactis (12,18%), L. mesenteroides subsp. mesenteroides (3,21%) e L. mesenteroides subsp. dextranicum (1,92%). Os isolados de Lactobacillus foram classificados como L. plantarum (8,97%), L. alimentarius (0,64%) e L. maltaromicus (0,64%). Os isolados de Streptococcus foram classificados como S. agalactiae (0,64%), S. mitior (0,64%) e S. parvulus (0,64%).
Title in English
Isolation and identification by numerical taxonomy of the raw milk lactic microbiota
Abstract in English
Samples of raw milk, were collected between May of 1990 and January of 1991, at the Colina dairy industry, district of Artemis, Piracicaba - SP, to determine the bacterial microbiota from milk. Plate counts were made in APT-S and selective medium for Leuconostoc, and 156 culture were selected for to be identified by the use of probability matrices in a computation program. The genus Lactococcus were the microorganisms most frequent of the lactic microbiota (42,31%), followed by genus Pediococcus (28,20%), Leuconostoc (17,31%), Lactobacillus (10,26%) and Streptococcus (1,92%). The Lactococcus strains were classified as L. raffinolactis (22,44%) and L. lactis subsp. lactis (19,87%). The classification of Pediococcus showed to be P. inopinatus (28,21%). The Leuconostoc strains were classified as L. lactis (12,18%), L. mesenteroides subsp. mesenteroides (3,21%) and L. mesenteroides subsp. dextranicum (1,92%). The Lactobacillus strains were classified as L. plantarum (8,97%), L. alimentarius (0,64%) and L. maltaromicus (0,64%). The Streptococcus strains were classified as S. agalactiae (0,64%), S. mitior (0,64%) and S. parvulus (0,64%).
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