Este estudo visou analisar a qualidade microbiológica do leite humano cru recebido em um banco de leite humano (BLH), quantificando os microrganismos aeróbios mesófilos, Staphylococcus coagulase positivos (ECP), coliformes totais e Escherichia coli. Foram realizadas análises microbiológicas em 60 amostras de leite humano cru recebidas no BLH e os resultados foram os seguintes: o microrganismo E. coli foi positivo em 50% das amostras analisadas, os coliformes totais em 75% delas, o ECP em 46,66% e em 96,66% do total das amostras foram detectados microrganismos aeróbios mesófilos. Das amostras analisadas, a população de E. coli foi positiva em 93,33% (28 amostras) com uma variação entre 1 e 99 NMP/mL; 3,33% (1 amostra) entre 10⁴ e 9,9 x 10⁴ NMP/mL; 3,33% (1 amostra) entre 10⁵ e 9,9 x10⁵ NMP/mL. A população de coliformes totais que foi positiva em 57,77% (26 amostras) das amostras analisadas ficou entre 1 e 99 NMP/mL; 24,4% (11 amostras) ficaram entre 10² e 9,9x10² NMP/mL; 2,22% (1 amostra) entre 10³ e 9,9x10³ NMP/mL; 8,88% (4 amostras) entre 10⁴ e 9,9x10⁴ NMP/mL; 4,44% (2 amostras) entre 10⁵ e 9,9x10⁵ NMP/mL; 2,22% (1 amostra) entre 10⁶ e 9,9x10⁶ NMP/mL. Os microrganismos aeróbios mesófilos apresentaram-se na quase totalidade das amostras, sendo que 17,24% (10 amostras) variaram de 1 a 99 UFC/mL; 17,24% (10 amostras) entre 10² e 9,9x10² UFC/mL; 10,34% (6 amostras) ficaram entre 10³ e 9,9 x 10³ UFC/mL; 25,86% (15 amostras) entre 10⁴ e 9,9 x10⁴ UFC/mL; 17,24% (10 amostras) entre 10⁵ e 9,9 x 10⁵ UFC/mL; 10,34% (6 amostras) entre 10⁶ e 9,9x10⁶ UFC/mL e 1,72% (1 amostra) entre 10⁷ e 9,9x10⁷ UFC/mL. Porém, 3,33% (2 amostras) apresentaram-se negativas. Do total de amostras analisadas, o grupo dos microrganismos ECP apresentouse em 78,57% (22 amostras) com variação entre 1 e 99 UFC/mL; em 3,57% (1 amostra) entre 10² e 9,9x10² UFC/mL; 10,71% (3 amostras) entre 10³ e 9,9x10³ UFC/mL e 7,14% (2 amostras) entre 10⁴ e 9,9 x 10⁴ UFC/mL.

This paper aimed to analyze the microbiological quality of the raw human milk which is received in a human milk bank (HMB) by quantifying the aerobic mesophiles, Staphylococcus aureus (SCP), total coliforms and Escherichia coli. Sixty (60) samples of raw human milk received in HMB were microbiologic analyzed and the results were as follows: the E. coli microorganism was positive in 50% of the analyzed samples, the total coliforms were in 75% of them, the ECP in 46,6% and in 96,6% of the total amount of samples aerobic mesophiles microorganisms were detected. From the total analyzed samples, the population of E. coli was positive in 93,33% (28 samples) with a variation between 1 and 99 NMP/mL; 3,33% (1 sample) was between 10⁴ and 9,9x10⁴ NMP/mL; 3,33% (1 sample) was from 10⁵ to 9,9x10⁵ NMP/mL. The population of the total coliforms which was positive in 57,77% (26 samples) of the total analyzed samples were between 1 and 99 NMP/mL; 24,4% (11 samples) were between 10² and 9,9x10² NMP/mL; 2,22% (1 sample) were between 10³ and 9,9x10³ NMP/mL; 8,88% (4 samples) were between 10⁴ and 9,9x10⁴ NMP/mL; 4,44% (2 samples) between 10⁵ and 9,9x10⁵ NMP/mL and in 2,22% (1 sample) from 10⁶ to 9,9x10⁶ NMP/mL. The aerobic mesophiles microorganisms were presented in almost all the samples, being: 17,24% (10 samples) with a variation of 1 to 99 UFC/mL; 17,24% (10 samples) between 10² and 9,9x10² UFC/mL; 10,34% (6 samples) were between 10³ and 9,9x10³ UFC/mL; 25,86% (15 samples) between 10⁴ and 9,9x10⁴ UFC/mL; 17,24% (10 samples) between 10⁵ and 9,9x10⁵ UFC/mL; 10,34% (6 samples) between 10⁶ and 9,9x10⁶ UFC/mL and in 1,72% (1 sample) between 10⁷ and 9,9x10⁷ UFC/mL. However 3,33% (2 samples) showed to be negative. From the total analyzed samples, the SCP group presented in 78,57% (22 samples) with a variation between 1 and 99 UFC/mL; 3,57% (1 sample) between 10² and 9,9 x 10² UFC/mL; 10,71% (3 samples) between 10³ e 9,9x10³ UFC/mL and 7,14% (2 samples) were between 10⁴ and 9,9x10⁴ UFC/mL.