• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Master's Dissertation
DOI
Document
Author
Full name
Jéssica Casarotto
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 2019
Supervisor
Committee
Sposito, Marcel Bellato (President)
Kluge, Ricardo Alfredo
Rodrigues, Paulo Hercílio Viegas
Sampaio, Aloisio Costa
Title in Portuguese
Conservação in vitro de morangueiros sob diferentes espectros de luz
Keywords in Portuguese
Fragaria x ananassa
LEDs
Micropropagação
Plântulas
Abstract in Portuguese
Os diodos emissores de luz (LEDs) são utilizados em vários setores da agricultura, principalmente no cultivo in vitro, por ser uma alternativa de fonte de luz mais eficiente. Além de proporcionar uma significativa economia de energia elétrica, pode também auxiliar no desenvolvimento das plantas. Algumas técnicas de cultivo in vitro tem a finalidade de conservar os recursos fitogenéticos, que são fundamentais para viabilizar o estabelecimento de bancos de germoplasma e permitir a produção comercial de plantas micropropagadas. No Brasil, essa técnica já é usada para a propagação de plantas, no entanto ainda são poucos experimentos com a presença de LEDs. O objetivo do estudo foi avaliar o desenvolvimento de plântulas de morangueiro da cultivar Camarosa conservadas in vitro, sob a ação de diferentes espectros de luz. Os explantes de morangueiro foram mantidos em tubos de ensaio contendo 10 ml de meio de cultura semi-sólido MS, acrescido de 30 g de sacarose L-1; 1,8 g L-1 de Phytagel® e 0,1 mg L-1 de IBA (ácido idolbutírico), permanecendo in vitro por 28, 56, 84, 112 e 140 dias sob os tratamentos de LEDs de cor branca, mista (70% vermelha + 30% azul), vermelha e azul, sem ocorrência de subcultivos. Em cada intervalo de tempo, foram avaliadas a altura, peso fresco, número e comprimento das raízes, número de folhas, diâmetro do caule, teores de clorofila a e b. As plântulas obtidas foram aclimatizadas por 21 dias e avaliadas a taxa de sobrevivência das plantas, expressas em porcentagem, o número de folhas, o diâmetro do caule e a altura das plantas. O uso de LEDs de cor vermelha e mista contribuíram para o desenvolvimento das plântulas, já a luz azul reduziu o desenvolvimento das plântulas. Em relação aos pigmentos fotossintéticos, a utilização de LEDs não teve interferência em nenhum tratamento sob as plântulas. Na aclimatização, as plântulas sob efeitos das luzes vermelha e mista, tiveram um melhor resultado de sobrevivência em até três meses de conservação sem subcultivos.
Title in English
In vitro conservation of strawberries under different light spectra
Keywords in English
Fragaria x ananassa
LEDs
Micropropagation
Seedlings
Abstract in English
Light emitting diodes (LEDs) are used in many agriculture sectors, especially in vitro cultivation, because it is a more efficient light source alternative. In addition to providing significant energy savings, it can also help in the development of plants. Some techniques of in vitro cultivation have the purpose of conserving plant genetic resources, which are fundamental to enable the establishment of germplasm banks and allow the commercial production of micropropagated plants. In Brazil, this technique is already used for the propagation of plants, however there are still few experiments with the presence of LEDs. The objective of the study was to evaluate the development of strawberry cv. Camarosa conserved in vitro, under the effect of different light spectra. The strawberry explants were kept in test tubes containing 10 ml of MS semi-solid culture medium, plus 30 g of sucrose L-1; 1.8 g L-1 of Phytagel® and 0.1 mg L-1 of IBA (idolbutyric acid), remaining in vitro for 28, 56, 84, 112 and 140 days under the treatments of mixed white LEDs (70% red + 30% blue), red and blue, without subculture interference. At each interval, height, fresh weight, number and length of roots, number of leaves, stem diameter, chlorophyll a and b contents were evaluated. The seedlings obtained were acclimatized for 21 days and evaluated the survival rate of the plants, expressed as percentage, number of leaves, stem diameter and height of plants. The use of red and mixed color LEDs contributed to the development of the seedlings, while the blue light reduced the development of the seedlings. In relation to the photosynthetic pigments, the use of LEDs did not interfere in any treatment under the seedlings. In acclimatization, the seedlings under the effects of red and mixed lights, had a better survival result in up to three months of conservation without subcultures.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
There are withheld file due to requirements (data publishing, patents or rights).
Release Date
2021-08-13
Publishing Date
2019-08-15
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
CeTI-SC/STI
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2021. All rights reserved.