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Master's Dissertation
DOI
https://doi.org/10.11606/D.11.1999.tde-20220208-042439
Document
Author
Full name
Rogério Biaggioni Lopes
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 1999
Supervisor
Title in Portuguese
Seleção de fungos entomopatogênicos e controle de Frankliniella occidentalis (Thysanoptera: Thripidae)
Keywords in Portuguese
CONTROLE BIOLÓGICO
FUNGOS ENTOMOPATOGÊNICOS
SELEÇÃO
TRIPES
Abstract in Portuguese
No presente estudo desenvolveu-se um método de criação de Frankliniella occidentalis sobre Canavalia ensiformis, com o objetivo de fornecer insetos para estudos de biologia e controle da praga. Também avaliou-se a patogenicidade de 45 isolados de fungos entomopatogênicos para ninfas do tripes em laboratório, sob condições controladas (temperatura = 27±0,5 oC; umidade relativa = 98±1 %; fotofase = 12 horas). O fungo Metarhizium anisopliae mostrou-se altamente patogênico e virulento para a espécie, com média de 97% de mortalidade de ninfas após quatro dias para todos os isolados testados. Comprovou-se também a eficiência do isolado de M. anisopliae selecionado no controle de infestações naturais da praga em cultivos comerciais de alface hidropônico. A eficiência de controle atingiu 60%, seis dias após a primeira pulverização e nas parcelas que não receberam o tratamento com o patógeno observou-se um aumento populacional de 46,5%. O sistema de criação de F. occidentalis mostrou-se adequado, produzindo de forma prática um grande número de insetos para os bioensaios de laboratório. Foi possível selecionar isolados de M anisopliae com grande potencial para utilização no controle do tripes em cultivos protegidos.
Title in English
Selection of entomopathogenic fungi in the control of Frankliniella occidentalis (Thysanoptera: Thripidae)
Abstract in English
ln the present study, a method of rearing Frankliniella occidentalis on Canavalia ensiformis was developed, with the objective of producing insects for studies on biology and control of the insect. Also, the pathogenicity of 45 isolates of entomopathogenic fungi to nymphs of the thrips F. occidentalis was evaluated in laboratory, under controlled conditions (temperature = 27±0.SoC; relative umidity = 98±1%; fotophase = 12 hours). All tested isolates of Metarhizium anisopliae were highly pathogenic and virulent to this species of thrips, causing an average of 97% mortality of nymphs four days after inoculation. One isolate of M. anisopliae was selected for tests of efficiency in the control of natural infestations of the insect in commercial areas of hidroponic lettuce. Six days after the :6.rst application, the e:fficiency of control was 60%. ln the areas that did not receive the treatment with the pathogen a population increase of 46.5% was observed. The technique for rearing F. occidentalis developed in this study was proven to be feasible way of obtaining a large number ofthrips for laboratory assays. It was possible to select an isolate of M. anisopliae with great potential for the control of the thrips in greenhouse.
 
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Publishing Date
2022-02-08
 
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