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Doctoral Thesis
DOI
10.11606/T.17.2019.tde-08012019-154153
Document
Author
Full name
Antonio Gustavo Zampar
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2018
Supervisor
Committee
Farina Junior, Jayme Adriano (President)
Carvalho, Viviane Fernandes de
Coltro, Pedro Soler
Frade, Marco Andrey Cipriani
Milcheski, Dimas André
Title in Portuguese
Análise de células mesenquimais multipotentes derivadas de diferentes áreas doadoras de tecido adiposo e sua influência sobre fibroblastos in vitro
Keywords in Portuguese
Anemia falciforme ; Células mesenquimais multipotentes ; Células tronco ; Fibroblastos ; Tecido adiposo ; Úlceras falciformes
Abstract in Portuguese
A cicatrização de feridas crônicas e de defeitos complexos representam desafios para a cirurgia plástica reconstrutiva. Novos tratamentos emergiram com a utilização de células tronco mesenquimais, com especial interesse para as derivadas de tecido adiposo (CTMs-TA), por possuir algumas vantagens em relação às derivadas da medula óssea. Algumas doenças poderiam se beneficiar com o uso das CTMs-TA, em particular, as feridas de pacientes portadores de anemia falciforme que ainda representam um desafio terapêutico. Neste estudo, investigou-se a existência de possíveis áreas doadoras preferenciais de CTMs-TA no tecido adiposo (TA) por meio da comparação de aspectos qualitativos e quantitativos das CTMs-TA derivadas de cinco diferentes áreas corporais. Posteriormente, analisou-se a influência do sobrenadante dessas células, rico em citocinas e fatores de crescimento, sobre a migração de fibroblastos de indivíduos normais e de portadores de anemia falciforme in vitro. Não foram observadas diferenças qualitativas entre as CTMs-TA das cinco áreas analisadas. A região do dorso apresentou número maior de CTMs, com diferença significativa em relação à região das coxas. A adição de sobrenadante produzido por CTMs-TA demonstrou aumento da velocidade de migração dos fibroblastos de forma similar para os normais e os falciformes. O microambiente desfavorável presente nas feridas falciformes parece exercer importante influência sobre esses fibroblastos, pois uma vez corrigido o microambiente com os meios de cultivo apropriados, as células apresentaram taxa de duplicação e velocidade de migração semelhante à dos fibroblastos normais in vitro.
Title in English
Analysis of multipotent mesenchymal cells derived from different adipose tissue donor areas and their influence on fibroblasts in vitro
Keywords in English
Adipose derived stem cells ; Adipose tissue ; Fibroblasts ; Multipotent mesenchymal cells ; Sickle cell anemia ; Stem cells
Abstract in English
The healing of chronic wounds and complex defects represents challenges for reconstructive plastic surgery. New treatments have emerged with the use of multipotent mesenchymal cells, with special interest for the adipose-derived stem cells (ADSCs) as it has some advantages over the bone marrow-derived. Some diseases could benefit from the use of ADSCs, in particular, the wounds of patients with sickle cell anemia that still represent a therapeutic challenge. In this study, we investigated the existence of possible preferential donor areas of ADSCs in adipose tissue by comparing qualitative and quantitative aspects of ADSCs derived from five different body areas. Later, we analyzed the influence of the supernatant of these cells, rich in cytokines and growth factors, on the migration of fibroblasts from healthy individuals and from patients with sickle cell anemia in vitro. No qualitative differences were observed among the ADSCs of the five areas analyzed. The dorsum presented a higher number of ADSCs, with a significant difference in relation to the thigh. Addition of supernatant produced by ADSCs has been shown to increase the rate of migration of fibroblasts in a similar way to healthy and sickle cells. The unfavorable microenvironment present in sickle wounds seems to exert a significant influence on these fibroblasts because once the microenvironment was corrected with the appropriate culture media, the cells had a doubling rate and migration rate similar to normal fibroblasts in vitro.
 
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Publishing Date
2019-02-15
 
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