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Master's Dissertation
DOI
10.11606/D.17.2018.tde-29032018-094554
Document
Author
Full name
Ana Carolina Tagliatti Zani
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2017
Supervisor
Committee
Silva, Julio Cesar Rosa e (President)
Gomes, Mariana Kefalás Oliveira
Lovato, Juliana Meola
Title in Portuguese
Influência do agente antiangiogênico bevacizumab em endometriose experimentalmente induzida em ratas
Keywords in Portuguese
Angiogênese
Bevacizumab
Diferenciação Celular
Endometriose
Invasão Tecidual
Proliferação Celular
Ratas
VEGF
Abstract in Portuguese
A endometriose, caracterizada por crescimento de tecido endometrial fora da cavidade uterina, é responsável por sintomas álgicos com grande impacto na qualidade de vida da paciente. Várias linhas de medicações têm sido estudadas para essa o tratamento da endometriose, já que a cirurgia não é o tratamento de escolha sempre e, quando realizado, não é um tratamento definitivo. O conhecimento da patogenia da endometriose é fundamental para o estudo de novas classes de medicações. Uma delas, os fatores inibitórios da angiogênese, tem papel fundamental no estabelecimento e crescimento de lesões de endometriose. Neste estudo, buscamos a influência da Bevacizumab, droga anti-fator de crescimento endotelial (anti-VEGF), utilizada em duas dosagens diferentes, em endometriose peritoneal induzida em ratas, modelo animal já bem estabelecido para o estudo de endometriose. As ratas permaneceram sob tratamento durante 4 semanas, após as quais foram sacrificadas, sendo as lesões e o corno uterino remanescente retirados para posterior avaliação. Foi realizada avaliação da área das lesões de cada rata, da presença de tecido endometrial à microscopia, da positividade para o anticorpo antiVEGF na imunohistoquímica e da expressão gênica de PCNA, MMP9, Tp63 e VEGFA. O bevacizumab atuou reduzindo a área das lesões nos grupos que receberam medicação (p=0,002) e reduzindo a expressão gênica para Tp63 nas lesões (p=0,04). Não houve resultado significativo nas outras avaliações
Title in English
Influence of antiangiogenic agent Bevacizumab on endometriosis experimentally induced in rats
Keywords in English
Angiogenesis
Bevacizumab
Cell Differentiation
Cell Proliferation
Endometriosis
Rats
Tissue Invasion
VEGF
Abstract in English
Endometriosis, characterized by growth of endometrial tissue outside the uterine cavity, is responsible for painful symptoms with great impact on the quality of life of women. Several lines of medications have been studied for endometriosis's treatment, since surgery is not always the treatment of choice and, when done, it is not a definitive treatment. Knowing the pathogenesis of this disease is fundamental for the study of new classes of medications. One of them, the inhibitory factors of angiogenesis, plays a fundamental role in the establishment and growth of endometriosis lesions. In this study, we sought the influence of Bevacizumab, an anti-endothelial growth factor (anti-VEGF) drug, used in two different dosages, in peritoneal endometriosis induced in rats, an animal model well established for the study of endometriosis. The rats remained under treatment for 4 weeks, after which they were sacrificed, with the remaining lesions and uterine horn being removed for further evaluation. An evaluation of the lesion area of each rat, the presence of endometrial tissue under microscopy, the positivity of the anti-VEGF antibody in immunohistochemistry and the gene expression of PCNA, MMP9, Tp63 and VEGFA were performed. Bevacizumab worked by reducing the area of the lesions in the groups receiving medication (p = 0.002) and reducing the gene expression for Tp63 in the lesions (p = 0.04). There was no significant result in the other evaluations
 
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Publishing Date
2018-07-19
 
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