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Doctoral Thesis
DOI
10.11606/T.18.2008.tde-01022009-165147
Document
Author
Full name
Leonardo Henrique Soares Damasceno
Institute/School/College
Knowledge Area
Date of Defense
Published
São Carlos, 2008
Supervisor
Committee
Zaiat, Marcelo (President)
Oliveira, Roberto Alves de
Ratusznei, Suzana Maria
Rodrigues, José Alberto Domingues
Santaella, Sandra Tedde
Title in Portuguese
Degradação do aldicarbe em biorreator anaeróbio horizontal de leito fixo
Keywords in Portuguese
Biorremediação
Carbamato
HPLC
Pesticida
RAHLF
Reator biológico
Abstract in Portuguese
O objetivo principal deste trabalho foi avaliar o desempenho do biorreator anaeróbio horizontal de leito fixo (RAHLF) na degradação de aldicarbe em condições anaeróbias. Foram avaliados três níveis de oxidação: metanogênico, sulfetogênico e desnitrificante. Inicialmente foi desenvolvido o método de detecção de aldicarbe e metabólitos por meio de cromatografia líquida de alta eficiência (HPLC) para amostras aquosas sem pré-tratamento. A validação por procedimentos estatísticos confirmou a viabilidade do mesmo. O efeito do aumento da concentração de aldicarbe no desempenho dos reatores foi avaliado pelo emprego das concentrações de 5, 10, 20, 30 e 40 mg/L de aldicarbe extraído do produto comercial. Os reatores foram submetidos ao tempo de detenção hidráulica de 24 horas. As melhores eficiências de remoção foram obtidas nas concentrações de 5 e 10 mg/L: 93,2 e 88,9% (metanogênico), 90,5 e 83,2% (sulfetogênico) e 88,0 e 94,3% (desnitrificante), respectivamente. De 20 a 40 mg/L, o aumento da concentração afluente de aldicarbe causou redução da eficiência de remoção em todos os níveis de oxidação avaliados. Os reatores metanogênico e sulfetogênico tiveram desempenho semelhante em todas as concentrações avaliadas, enquanto que o reator desnitrificante não foi adequado para concentrações superiores a 10 mg/L. Nos ensaios com reatores diferenciais na concentração de aldicarbe de 10 mg/L, verificou-se que, em condições metanogênicas, não houve influência significativa da resistência à transferência de massa externa e interna na velocidade global de conversão de aldicarbe. Nestas condições, os parâmetros cinéticos aparentes corresponderam aos parâmetros cinéticos intrínsecos. A constante cinética de 1ª ordem (k1), validada por meio do Teste F, foi de 1,46 '+ OU -' 0,09·'10 POT.-5'·L/mgSVT.h (r2=0,994 '+ OU -' 0,001). As análises de biologia molecular para o Domínio Bacteria constataram predominância de Chloroflexi e Epsilon proteobacterium. No Domínio Archaea houve predominância de Methanosaeta.
Title in English
Aldicarb degradation in a horizontal-flow anaerobic immobilized biomass bioreactor
Keywords in English
Biological reactor
Bioremediation
Carbamate
HAIB
HPLC
Pesticide
Abstract in English
A bench-scale horizontal-flow anaerobic mmobilized biomass (HAIB) bioreactor was assayed aiming to verify its potential use for aldicarb degradation. Three levels of oxidation were evaluated: methanogenic, sulfidogenic and denitrifying conditions. An HPLC method for the determination of aldicarb, aldicarb sulfoxide and aldicarb sulfone in liquid samples without pretreatment was developed and validated. The effects of increasing aldicarb concentration were evaluated at 5, 10, 20, 30 and 40 mg/L, extracted from the commercial product. The bioreactors were operated at a constant hydraulic detention time of 24 hours and 30°C. The best-removal efficiencies were obtained at the concentrations of 5 and 10 mg/L: 93.2 and 88.9% (methanogenic), 90.5 and 83.2% (sulfidogenic) e 88.0 and 94.3% (denitrifying), respectively. From 20 to 40 mg/L, the increase at the concentration of aldicarb caused reduction in the efficiency of removal in all levels of oxidation evaluated. The methanogenic and sulfidogenic bioreactors had similar performance, while the denitrifying bioreactor was not appropriate for concentrations above 10 mg/L. In the assays with differential reactors at the aldicarb concentration of 10 mg/L, the external and internal mass transfer resistance did not affect the overall substrate utilization rates. Thus, in these conditions, the apparent kinetic parameters corresponded to the intrinsic kinetic parameters. The first order rate constant (k1), validated through F-test, was 1.46 '+ OU -' 0.09·'10 POT.-5'·L/mgSVT.h (r2=0.994 '+ OU -' 0.001). The analysis of molecular biology for the Bacteria Domain showed predominance of Chloroflexi and Epsilon proteobacterium. In the Archaea Domain the predominant microrganism was Methanosaeta.
 
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Publishing Date
2009-02-16
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • DAMASCENO, L H S, et al. Development and Validation of a HPLC Method for the Determination of Aldicarb, Aldicarb Sulfoxide and Aldicarb Sulfone in Liquid Samples from Anaerobic Reactors [doi:10.1590/S0103-50532008000600016]. Journal of the Brazilian Chemical Society [online], 2008, vol. 19, p. 1158-1164.
  • DAMASCENO, Leonardo H. S., et al. Development and validation of a HPLC method for the determination of aldicarb, aldicarb sulfoxide and aldicarb sulfone in liquid samples from anaerobic reactors [doi:10.1590/S0103-50532008000600016]. Journal of the Brazilian Chemical Society [online], 2008, vol. 19, n. 6, p. 1158-1164.
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