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Master's Dissertation
DOI
10.11606/D.23.2004.tde-21012005-105725
Document
Author
Full name
Fernanda Campos Sousa de Almeida
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2004
Supervisor
Committee
Nunes, Fabio Daumas (President)
Durazzo, Marcelo Doria
Rosa, Luiz Eduardo Blumer
Title in Portuguese
Localização dos transcritos dos genes WNT5A e HOXB5 em carcinomas epidermóides de boca através da técnica de hibridização “in situ”.
Keywords in Portuguese
câncer de boca
carcinoma epidermóide
hibridização
RT-PCR
Abstract in Portuguese
São freqüentes alterações em vias de sinalização de genes de desenvolvimento, no que diz respeito ao carcinoma epidermóide de boca (CEB). Vinte e nove casos de CEB e tecido não tumoral adjacente à neoplasia foram investigados através da técnica de hibridização “in situ”. Os transcritos dos genes WNT5A e HOXB5 foram observados em todos os casos de tumor. A hibridização “in situ” revelou que o WNT5A estava mais expresso em tumores bem diferenciados. Adicionalmente, observou-se transcritos do WNT5A em glândulas salivares menores, estroma glandular, estroma tumoral e alguns vasos sanguíneos Entretanto, com respeito ao HOXB5 não foi possível estabelecer mudança do padrão do transcrito nos diferentes graus histológicos nas amostras de CEB. O HOXB5 também pôde ser identificado em alguns fragmentos de glândulas salivares menores, tecido muscular e em endotélio. Os resultados do presente estudo sugerem que a expressão dos genes WNT5A e HOXB5 podem estar relacionadas com a diferenciação e progressão do câncer de boca.
Title in English
WNT5A e HOXB5 localization study in oral squamous cell carcinoma with in situ hybridization.
Keywords in English
homeobox
HOXB5
oral cancer
Squamous cell carcinoma
WNT5A – RT-PCR- In situ hybridization
Abstract in English
Disruption in developmental genes pathway are common in oral squamous cell carcinoma (OSCC). This study investigated the pattern of expression of two developmental genes, WNT5A and HOXB5, in 29 cases of OSCC and adjacent non tumoural tissue using in situ hybridization technique. Transcripts for WNT5A and HOXB5 were detected in all tumoral samples. In situ hybridization technique demonstrated that WNT5A transcripts were mainly detected in well differentiated tumors when compared with moderately and undifferentiated OSCC. WNT5A transcripts were also observed in accessory salivary glands, glandular stroma, and vessels. Therefore, for the HOXB5 transcript it was not possible to stability a relationship with the tumoral histological grade. The expression of HOXB5 transcripts in non tumoral samples was detected in salivary glands, glandular stroma, endothelium, and muscle. Results suggest that WNT5A and HOXB5 genes play a possible role in tumor differentiation and cancer progression.
 
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FernandaTeseToda.pdf (1.38 Mbytes)
Publishing Date
2005-02-02
 
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