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Master's Dissertation
DOI
10.11606/D.25.2007.tde-15102007-092541
Document
Author
Full name
Érika Sinara Lenharo Orti-Raduan
Institute/School/College
Knowledge Area
Date of Defense
Published
Bauru, 2007
Supervisor
Committee
Taveira, Luis Antônio de Assis (President)
Lara, Vanessa Soares
Nascimento, Dejair Caitano do
Title in Portuguese
Análise quantitativa das células de Langerhans em mucosa bucal de pacientes submetidos ao transplante de medula óssea alogênico com doença enxerto contra hospedeiro crônica
Keywords in Portuguese
Células de Langerhans
Leucemia
Transplante alogênico de medula óssea
Abstract in Portuguese
A doença enxerto contra hospedeiro é uma complicação comum nos pacientes submetidos ao transplante de medula óssea alogênico. Com o objetivo de contribuir para o esclarecimento da participação das células de Langerhans na doença enxerto contra hospedeiro crônica (GVHDc) quando de sua ocorrência na mucosa bucal, foram analisados 40 pacientes oncohematológicos e hematológicos submetidos ao transplante de medula óssea alogênico no Hospital Amaral Carvalho, Jaú - SP. Cortes microscópicos de 3µm de espessura da mucosa jugal com padrão de normalidade (controle - 20 pacientes) e de pacientes transplantados com e sem GVHDc, foram avaliados em hematoxilina e eosina e pela técnica imuno-histoquímica padrão da estreptavidina-biotina-peroxidase utilizando-se o anticorpo monoclonal anti- CD1a. As células de Langerhans imunomarcadas foram quantificadas no epitélio da mucosa jugal, sendo o número médio destas células estatisticamente comparado entre os pacientes controle e os pacientes transplantados com e sem GVHDc. Os resultados demonstraram um maior número de células de Langerhans na mucosa jugal dos pacientes com GVHDc quando comparado aqueles sem GVHDc e ao grupo controle (p=0,001). Foi observado também, a presença de intenso infiltrado inflamatório crônico, justaepitelial, com desorganização das células da camada basal do epitélio, com vacuolização celular, satelitose, corpúsculos acidofílicos e ocorrência de clivagem entre e o epitélio e o tecido conjuntivo nos pacientes que desenvolveram GVHDc. Estes resultados sugerem que a células de Langerhans participa da doença enxerto contra hospedeiro crônica na mucosa jugal dos pacientes submetidos ao transplante de medula óssea alogênico, sendo provavelmente recrutadas pelo processo inflamatório e imunopatológico que caracteriza esta doença.
Title in English
Quantitative analysis of Langerhans cells in oral mucosa of patients treated with allogeneic bone marrow transplantation with chronic graft versus host disease
Keywords in English
Bone marrow transplantation
Langerhans cells
Leukemia
Abstract in English
The graft versus host disease (GVHD) is a common complication in patients submited to alogeneic bone marrow transplantation. To understand the role of Langerhans cells in chronic GVHD (cGVHD) in oral mucosa, we analyzed 40 oncohematological or hematological patients who received alogeneic bone marrow transplantation at Hospital Amaral Carvalho, Jaú - SP. Slices of 3µm from normal oral mucosa (control - 20 patients) and transplanted patients with and without cGVHD were analyzed by hematoxylin-eosin technique and conventional immunohistochemistry of streptavidin-biotin peroxidase technique for monoclonal antibody anti-CD1a. The immunomarked Langerhans cells were quantified in the epithelium of oral mucosa; the average number of these cells was statistically significant when compared to the control group and patients with and without cGVHD. The results showed higher number of Langerhans cells in oral mucosa of cGVHD when we compared the control group and the group of patients with and without cGVHD (p=0,001). We also observed the presence of chronic juxtaepithelial inflammatory infiltrate, with basal layer epithelium desorganization, vacuolization, satellitosis, acidophilic bodies and presence of gap between epithelium and connective tissue of patients with cGVHD. These results suggest that Langerhans cells may have a role in cGVHD of oral mucosa in patients submited to alogeneic bone marrow transplantation, and they may be recruited by inflammatory and immunopathologic process that are characteristic in this disease.
 
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ErikaOrtiRaduan.pdf (2.04 Mbytes)
Publishing Date
2007-10-15
 
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