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Master's Dissertation
DOI
10.11606/D.41.2019.tde-18022019-091636
Document
Author
Full name
Ana Maria Pereira Barreto Amorim
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2018
Supervisor
Committee
Ho, Fanly Fungyi Chow (President)
Jimenez, Paula Christine
Lotufo, Leticia Veras Costa
Title in Portuguese
Estudo de antioxidantes, potencial bioativo e composição química de Chnoospora minima, Dictyopteris plagiogramma, Padina gymnospora, Sargassum cymosum (Ochrophyta) e Codium isthmocladum (Chlorophyta)
Keywords in Portuguese
Alga verde
Algas pardas
Antioxidante
Atividades biológicas
Prospecção
Abstract in Portuguese
As macroalgas marinhas constituem uma importante fonte de produtos naturais, devido à sua composição química, rica em substâncias fenólicas, terpenos e polissacarídeos sulfatados, apresentando potencial biotecnológico como fonte de substâncias com atividade antioxidante, antiviral, antitumoral e antibacteriana. Nesse sentido, o objetivo do presente estudo foi avaliar o potencial de prospecção de macroalgas do litoral do Espírito Santo (ES), mediante estudos dos potenciais antioxidante e bioativo de extratos brutos de quatro espécies de algas pardas (Chnoospora minima, Dictyopteris plagiogramma, Padina gymnospora e Sargassum cymosum) e de uma espécie de alga verde (Codium isthmocladum). A caracterização inicial dos extratos acetato de etila e metanólico das algas pardas, demonstrou a presença de constituintes com características distintas, indicando que pertencem à diferentes classes fenólicas. Os extratos metanólicos e aquosos das cinco espécies apresentaram os maiores rendimentos, sendo utilizados para análise do potencial antioxidante e bioativo. O extrato metanólico de P. gymnospora apresentou o maior potencial antioxidante e o maior teor de substâncias fenólicas totais pelo ensaio Folin-Ciocalteu, indicando o potencial dessa espécie como ingrediente funcional e na busca de substâncias antioxidantes naturais. As espécies analisadas apresentaram potencial bioativo (antiviral e antitumoral), sendo que os extratos aquosos foram mais ativos, quando comparados aos metanólicos. O maior potencial antiviral foi encontrado nos extratos aquosos de P. gymnospora, S. cymosum e C. isthmocladum, e o maior potencial antitumoral nos extratos aquosos de D. plagiogramma e C. isthmocladum. Tanto o potencial antiviral, como antitumoral podem estar relacionados à presença de substâncias fenólicas e polissacarídeos sulfatados nos extratos. Os resultados obtidos demonstram que as espécies estudadas, principalmente D. plagiogramma, P. gymnospora, S. cymosum e C. isthmocladum, apresentam potencial para prospecção de produtos naturais
Title in English
Study of antioxidants, bioactive potential and chemical composition of Chnoospora minima, Dictyopteris plagiogramma, Padina gymnospora, Sargassum cymosum (Ochrophyta) and Codium isthmocladum (Chlorophyta)
Keywords in English
Antioxidant
Biological activities
Brown seaweeds
Green seaweed
Prospection
Abstract in English
Marine macroalgae are an important source of natural products, due to their chemical composition, rich in phenolic substances, terpenes and sulphated polysaccharides, presenting biotechnological potential as a source of substances with antioxidant, antiviral, antitumor and antibacterial activity. In this sense, the objective of the present study was to evaluate the potential of macroalgae prospecting on the coast of Espírito Santo (ES), through studies of the antioxidant and bioactive potentials of crude extracts of four species of brown seaweeds (Chnoospora minima, Dictyopteris plagiogramma, Padina gymnospora and Sargassum cymosum) and a species of green seaweed (Codium isthmocladum). The initial characterization of the ethyl acetate and methanolic extracts of the brown seaweeds, showed the presence of constituents with distinct characteristics, indicating that they belong to the different phenolic classes. The methanolic and aqueous extracts of the five species presented the highest yields, being used for analysis of the antioxidant and bioactive potential. The methanolic extract of P. gymnospora presented the highest antioxidant potential and the highest content of total phenolic substances by the Folin-Ciocalteu assay, indicating the potential of this species as a functional ingredient and the search for natural antioxidant substances. The analyzed species presented bioactive potential (antiviral and antitumor), and the aqueous extracts were more active when compared to methanolic. The highest antiviral potential was found in aqueous extracts of P. gymnospora, S. cymosum and C. isthmocladum, and the highest antitumor potential in aqueous extracts of D. plagiogramma and C. isthmocladum. Both antiviral and antitumoral potential may be related to the presence of phenolic substances and sulfated polysaccharides in the extracts. The results showed that the studied species, especially D. plagiogramma, P. gymnospora, S. cymosum and C. isthmocladum, present potential for the prospection of natural products
 
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Release Date
2021-02-17
Publishing Date
2019-03-08
 
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