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Doctoral Thesis
DOI
10.11606/T.42.2013.tde-06062014-124909
Document
Author
Full name
Bruna Cecilia Caixeta de Oliveira
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2013
Supervisor
Committee
Liberti, Edson Aparecido (President)
Azeredo, Rogerio Albuquerque
Mori, Matsuyoshi
Oda, Margareth
Picosse, Luis Ronaldo
Title in Portuguese
Distribuição do IGF-I e do seu receptor na cartilagem do processo condilar da mandíbula e na sincondrose basiesfenoidal de ratos wistar subnutridos.
Keywords in Portuguese
Cartilagem
Côndilo mandibular
Crânio
Desnutrição
Fatores de crescimento
Mandíbula
Abstract in Portuguese
A cartilagem do processo condilar (PC) e a sincondrose basiesfenoidal (SB) participam do processo de crescimento e desenvolvimento craniofacial que são determinados pelo aporte protéico, pela ação hormonal e por fatores de crescimento, sendo o IGF-I o principal deles. Objetivou-se correlacionar as alterações morfológicas no PC e na SB provenientes da subnutrição protéica. Os grupos experimentais foram formados por animais heterogêneos (n=5) com 60 dias de vida, de acordo com o teor de caseína contida nas rações, protéica (20%) ou hipoprotéica (5%), formando, respectivamente, os grupos nutrido (N) e subnutrido (S). Na microscopia de luz foi observado que a subnutrição não alterou as espessuras das camadas do PC e da SB, enquanto que através da imunohistoquímica o número de IGF-I e IGF-IR diminuiu em ambos os tecidos (N≠S; p<0,05). No PC, o colágeno do tipo I passou a ser do tipo II no grupo S, enquanto que na SB, o do tipo II foi destacado em ambos os grupos. A matriz extracelular do PC apresentou-se densa e com coloração homogênea nos nutridos, contrastando com o aspecto difuso dos subnutridos. Na SB, tanto no grupo N quanto no S, a MEC manteve-se com aspecto uniforme na distribuição e na homogeneidade da coloração.
Title in English
Distribution of IGF-I and its receptor in the cartilage of the mandibular condyle process and basiesphenoidal synchondrosis of the undernourished wistar rats.
Keywords in English
Cartilage
Growth factors
Jaw
Malnutrition
Mandibular condyle
Skull
Abstract in English
The cartilage of the condylar process (CP) and the basiesfenoidal synchondrosis (BS) participate in the process of craniofacial growth and development that are determined by the protein content, the hormonal and growth factors, being the IGF-I main one. This study aimed to correlate the morphological changes in PC and SB from protein malnutrition. The experimental groups were formed by heterogeneous animals (n = 5) at 60 days of life, according to the casein contained in the feed, proteic (20%) or hypoproteic (5%), constituting respectively the nourished (N) and undernourished (U) groups. Under light microscopy it was observed that undernourished did not change the thickness of the layers of CP and BS by immunohistochemical while the number of IGFI and IGF-IR decreased in both tissues (N≠S; p<0,05). On the PC, the type I collagen became type II at the U group, while in the SB the type II was noted in both groups. The extracellular matrix (ECM) of the PC presented dense and homogenous coloration in the nourished, contrasting with the diffuse aspect of the undernourished. In SB, both in the N group as U, the ECM remained uniform in appearance and the distribution and uniformity of staining.
 
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Publishing Date
2014-06-06
 
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