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Master's Dissertation
DOI
Document
Author
Full name
Jéssica Catharine Gomes Duarte
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2017
Supervisor
Committee
Bittencourt, Jackson Cioni (President)
Horta Junior, José de Anchieta de Castro e
Lotfi, Claudimara Ferini Pacicco
Ortis, Fernanda
Title in Portuguese
A presença do hormônio concentrador de melanina no ovário no ciclo reprodutivo de ratas Sprague-Dawley.
Keywords in Portuguese
Western blotting
Imuno-histoquímica
MCH
MCH sérico
Neurohormônio
Abstract in Portuguese
No hipotálamo, centro regulador de uma variedade de comportamentos inclusive o reprodutivo, são produzidos diversos peptídeos moduladores, dentre eles o hormônio concentrador de melanina [MCH]. Já foi observada presença do MCH também em tecidos periféricos, incluindo os testículos. Por isso buscamos sua presença no ovário de ratas nas fases do ciclo estral, gestantes e lactantes. Para a determinação da presença do peptídeo foram utilizados os métodos de imuno-histoquímica e western blotting, para a detecção do RNAm, a hibridização in situ e RT-PCR, e ensaio imunoenzimático para determinação de níveis séricos hormonais de progesterona, prolactina e MCH. Nossos experimentos indicam a presença do RNAm do ppMCH no ovário, bem como do peptídeo em todas as fases do ciclo reprodutivo, no corpo lúteo e na medula ovariana, resultado quantificado pela técnica de western blotting. Além do mais, observamos que os níveis séricos de MCH são constantes nas fases analisadas e que a contribuição do ovário nesses níveis é significativa.
Title in English
The presence of melanin-concentrating hormone in the reproductive cycle in Sprague-Dawley rats ovary.
Keywords in English
Immunohistochemistry
MCH
Neurohormone
Serum MCH
Western blotting
Abstract in English
In the hypothalamus, the regulatory center of a variety of behaviors, including reproductive behavior, several peptides modulating these functions are produced, among them the melanin-concentrating hormone [MCH]. In addition to the brain tissue, its presence in several peripheral tissues, including the testis, has been observed. Therefore, we used rats to investigate the presence of MCH in the estrous cycle phase, during gestation and lactation periods. In order to determine the presence of the peptide, immunohistochemistry and western blotting methods were used. To detect the preproMCH RNAm, we used in situ hybridization and RT-PCR, and immunoenzymatic assay to determine serum levels of progesterone, prolactin and MCH. Our findings demonstrated the presence of ppMCH mRNA in ovary, as well as the peptide, and it was localized in the corpus luteum and ovarian marrow. Furthermore, we observed that serum MCH levels are constant in the analyzed phases and that the contribution of the ovary at these levels is significant.
 
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Release Date
2019-07-06
Publishing Date
2017-07-06
 
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