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Master's Dissertation
DOI
10.11606/D.42.2008.tde-13032009-111228
Document
Author
Full name
William Paganini Mayer
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2008
Supervisor
Committee
Liberti, Edson Aparecido (President)
Azeredo, Rogerio Albuquerque
Oliveira, Flavia de
Title in Portuguese
Características estruturais, ultra-estruturais e morfoquantitativas dos músculos tibial anterior e sóleo de ratos jovens submetidos à imobilização da articulação talocrural.
Keywords in Portuguese
Articulação talocrural
Fibras musculares
Imobilização
Órtese funcional
Ultra-estrutura
Abstract in Portuguese
Avaliou-se a tipificação das fibras musculares e determinou-se parâmetros morfoquantitativos dos efeitos da imobilização por 2 e 4 semanas. Após o período experimental, os músculos (mm.) foram corados por HE e Picro-sírius. Para a tipificação das fibras, utilizou-se as reações da ATPase e NADH-tr; a análise ultraestrutural procedeu-se com microscopia eletrônica de transmissão. Nos resultados, alguns mm. imobilizados (Im) exibiram núcleos centralizados e fibras tipo IIB com formas elusivas. O perimísio destes mm. se espessou, sendo constituído por fibras colágenas tipo III. Os fusos musculares apresentaram espessamento da cápsula. Houve alterações nas linhas Z e modificação de outras ultraestruturas como as mitocôndrias. A área de secção transversa dos mm. Im por 4 semanas foi menor. As fibras do tipo IIB reduziram a área de perfil e aumentaram a densidade, além disso, se ampliou a porcentagem das fibras do tipo IIA. Portanto, a imobilização promoveu alterações nos fusos e esqueleto fibroso muscular, assim como transformou fibras do tipo IIB em IIA.
Title in English
Structural, Ultrastructural and morphoquantitative features of tibials anterior and soleus muscle fibers of young rats subjected to talocruralis joint immobilization.
Keywords in English
Functional orthosis
Immobilization
Muscle Fibers
Talocruralis joint
Ultrastructure
Abstract in English
The effects of muscle immobilization for 2 and 4 weeks were studied by determining morphoquantitative parameters and muscle fiber-typing. After the trial period the muscles (mm.) were subject to HE and Picrosirius stain. The reactions of ATPase and NADH-tr were used for muscles fiber-typing. The ultrastructural analyses were made by transmission electron microscopy. The results showed changes in some of immobilized (Im) mm., it exhibited centralized nuclei cells and type IIB fibers took indefinite forms. The perimisium of Im mm. thicker up, and consisted of type III collagen fibers. Also the muscle spindles capsule thickened. Changes in the Z lines and other ultrastructures such as mitochondria were noticed. The cross-section area of Im mm. for 4 weeks was lower. The type IIB fibers reduced the profile area and density increased. Also the percentage of type IIA fibers expanded. Therefore the immobilization promoted changes in muscle spindles, fibrous skeleton morphology and provide an interconversion of muscle fibers type IIB into IIA.
 
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Publishing Date
2009-03-23
 
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