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Master's Dissertation
DOI
10.11606/D.42.2019.tde-18012019-132255
Document
Author
Full name
Ricardo Gabriel Oliveira Maia
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2018
Supervisor
Committee
Canteras, Newton Sabino (President)
Chacur, Marucia
Ribeiro, Fernando Augusto de Oliveira
Torrão, Andréa da Silva
Title in Portuguese
Análise eletrofisiológica multi-unitária da matéria cinzenta periaquedutal dorsal e camadas intermediárias e profundas do colículo superior de ratos durante ameaça predatória.
Keywords in Portuguese
Colículo Superior
Eletrofisiologia
Matéria Cinzenta Periaquedutal
Medo
Neurobiologia
Abstract in Portuguese
A circuitaria neuro-anatômica envolvida com a organização de respostas de medo inclui muitos sítios subcorticais diferentes, dois dos quais são a região dorsal da matéria cinzenta periaquedutal (dPAG) e as camadas intermediárias e profundas do colículo superior (i/dlSC). Este estudo investigou como as células destas duas regiões mudam de atividade em um rato que foi colocado diante de seu predador, o gato, comparado com outras situações onde o risco menor. 11 ratos wistar machos passaram por cirurgia para implantação de um microdriver contendo 8 tetrodos, que detectou 39 células na dPAG de 7 ratos e 44 células nas i/dlSC de 4 ratos. A atividade das células foi registrada em uma condição de base e em quatro condições experimentais distintas: o rato confinado, o rato diante de uma novidade, diante de um predador e diante do contexto predatório. Os dados coletados foram analisados por scripts de MATLAB e cada célula foi classificada para sua responsividade às condições experimentais, à mudança do comportamento do rato, à velocidade e à posição do rato no aparato. Na dPAG as células se mostraram mais responsivas a condição do gato do que as demais condições experimentais, tanto em número de células como em aumento de atividade celular. Uma quantidade menor de células mostrou-se responsiva a mudanças de comportamento, particularmente os comportamentos de defesa. Nas i/dlSC as células também se mostraram mais responsivas para o gato, seguido do contexto, em ambos os casos com diminuição de atividade celular, que foi mais intensa no gato. Uma quantidade menor de células pareceu responder de forma similar para as diferentes mudanças comportamentais. Interpretamos esses dados propondo que a dPAG possui uma função sinalizadora de medo, fortemente ativada durante o gato, e que as i/dlSC são inibidas diante do gato a fim de evitar a captação de estímulos irrelevantes para lidar com a situação de risco.
Title in English
Multi-unitary electrophysiological analysis of the dorsal periaqueductal gray and the intermediate and deep layers of the superior colliculus of rats during predatory threat.
Keywords in English
Electrophysiology
Fear
Neurobiology
Periaqueductal Gray
Superior Colliculus
Abstract in English
The neuroanatomical circuitry involved with the organization of fear responses includes a great number of subcortical sites, two of which are the dorsal periaqueductal gray (dPAG) and the intermediate and deep layers of the superior colliculus (i/dlSC). This study investigated how the cellular activity in these regions changed in a rat that was exposed to one of its predators, a cat, when compared with lower risk situations. 11 male wistar rats underwent surgery to implant a microdriver containing 8 tetrodes, which detected 39 cells in the dPAG of 7 rats and 44 cells in the i/dlSC of 4 rats. Cell activity was registered in a basal condition and then again in for experimental conditions: the rat confined in a smaller space, the rat facing a novel stimulus, the rat facing a cat and the rat facing the predatory context. Collected data was analyzed using MATLAB and each cell was classified according to its responsivity to the experimental conditions, to the rats switches in behavior, to the rats speed and position in the experimental apparatus. In the dPAG, cells were shown to be more responsive to the cat condition over the other conditions, both in terms of number of responsive cells and intensity of increase in firing activity. A smaller number of cells were responsive to behavior switching, being especially sensitive to the initiation of defensive behaviors. In the i/dlSC, cells were more responsive to the cat, followed by the predatory context, in both cases with a general reduction of cellular activity that was more intensive for the cat. A smaller number of cells was responsive in similar ways for behavior switching across experimental conditions. We interpret this data by proposing that the dPAG acts signaling a state of fear, particularly during a high risk situation, and the i/dlSC are inhibited during riskier situations so as to block irrelevant environmental stimuli.
 
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Release Date
2021-01-17
Publishing Date
2019-01-28
 
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