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Master's Dissertation
DOI
10.11606/D.42.2009.tde-06042009-164847
Document
Author
Full name
Daniela Higashi
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Simionato, Maria Regina Lorenzetti (President)
Jardim Junior, Elerson Gaetti
Marques, Marcia Martins
Title in Portuguese
Identificação de genes de isolados clínicos de Porphyromonas gingivalis expressos diferencialmente na formação de biofilme, usando differential display PCR.
Keywords in Portuguese
Porphyromonas gingivalis
Biofilmes
DD PCR
Expressão de genes
Abstract in Portuguese
Porphyromonas gingivalis é um bacilo anaeróbio Gram negativo envolvido com o início e progressão de doenças periodontais. É considerado um colonizador tardio ou secundário do biofilme oral capaz de aderir a células de Streptococcus gordonii, um colonizador inicial do biofilme dental. Este estudo se propôs a comparar a expressão de genes de amostras de P. gingivalis isoladas de diferentes condições periodontais usando Differential Display (DD) Reverse Transcription PCR, durante a formação de biofilme misto com S. gordonii. O perfil de expressão gênica de células em biofilme (saúde e periodontite) foi comparado, assim como com células planctônicas pareadas. Bandas diferencialmente expressas nas diferentes condições foram clonadas e seqüenciadas, seguida de identificação dos genes em bancos de dados. A confirmação da expressão diferencial dos genes detectados foi realizada através de PCR em Tempo Real. Desses genes, alguns se relacionam com fatores de virulência, outros com obtenção de energia além de genes relacionados com proteínas de membrana e de transporte.
Title in English
Identification of genes from Porphyromonas gingivalis differentially expressed in biofilm formation, using differential display PCR.
Keywords in English
Porphymonas gengivalis
Biofilms
DD PCR
Gene expression
Abstract in English
Porphyromonas gingivalis is a Gram negative anaerobic rod involved with the beginning and progression of periodontal diseases P. gingivalis is a late or secondary colonizer of oral biofilms and adhere to cells of Streptococcus gordonii, an early colonizer of dental plaque. This study aim to compare the gene expression of P. gingivalis strains isolated from different periodontal conditions using Differential Display (DD) Reverse Transcription PCR, in a mixed biofilm with S. gordonii. The gene expression profile was determined with biofilm cells (health and disease) and also with their planktonic samples partners. Bands differentially expressed were cloned, sequenciated and analyzed in gene data bank. Differential expression was confirmed by Real Time PCR. Some of the confirmed genes are related to virulence factors, energy metabolism and transport and membrane proteins.
 
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Publishing Date
2009-04-07
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • HIGASHI, D., et al. Gene expression of biofilm cells of clinical isolates of P. gingivalis. In 110th General Meeting, San Diego CA, 2010. Abstracts. : ASM, 2010. Abstract.
  • HIGASHI, D., et al. Genes from clinical isolates of Porphyromonas gingivalis are differentially expressed in biofilms. In 109th General Meeting, Philadelphia, 2009. Abstracts 109th General Meeting.Philadephia : ASM, 2009. Abstract.
All rights of the thesis/dissertation are from the authors
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