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Master's Dissertation
DOI
10.11606/D.42.2012.tde-09112012-115558
Document
Author
Full name
Luana de Cássia Umeda
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2012
Supervisor
Committee
Mehnert, Dolores Ursula (President)
Carmona, Rita de Cássia Compagnoli
Mucci, José Luiz Negrão
Title in Portuguese
Métodos clássicos e moleculares para avaliação da qualidade virológica de lodo de esgoto e de água de reúso: determinação da eficiência e limites de detecção.
Keywords in Portuguese
Adenovírus
Lodo de esgoto
Reúso da água
Rotavírus
Saneamento
Abstract in Portuguese
Os vírus entéricos humanos são encontrados no esgoto e em subprodutos dos processos de tratamento. Recentemente vem sendo recomendados como indicadores de qualidade microbiológica em normas da legislação brasileira e também nas de outros países, mas ainda com parâmetros a definir. O objetivo do estudo é a avaliação e a comparação entre métodos clássicos e moleculares aplicados à detecção de vírus entéricos em amostras de água de reúso e de lodo, visando subsidiar a legislação brasileira. Ensaios de semeadura experimental de protótipos de rotavírus e de adenovírus foram realizados nas matrizes ambientais e os vírus detectados por métodos clássicos (cultivo celular e reação de imunoperoxidase) e moleculares (PCR/nested-PCR, RT-PCR e ICC-PCR), determinando-se os limites de detecção de cada método para cada matriz. A pesquisa de rotavírus e adenovírus presentes naturalmente em 25 amostras de água de reúso e em 25 de lodo possibilitou a comparação dos métodos propostos. O ICC-PCR mostrou ser o método mais factível a ser aplicado na área de saneamento.
Title in English
Standard and molecular methods for surveillance of human enteric viruses in sludge and reclaimed water: efficiency and detections limits.
Keywords in English
Adenovirus
Reclaimed water
Rotavirus
Sanitation
Sludge
Abstract in English
Human enteric viruses are common contaminants of raw sewage and subproducts of sewage treatment processes. In recent years, those viruses were recommend as new microbiological indicators in different matrices in Brazilian legislation and others countries, although some questions should be elucidated. At present, the aim was to evaluate and compare the efficiencies of standard and molecular virological methods for detection of human enteric viruses in sludge and reclaimed water samples. Rotavirus and adenovirus were experimentally spiked in the proposed matrices and virus recovery and detection limits established for each method and matrice. Viruses naturally presented in 25 samples of sludge and 25 samples of reclaimed water were assayed by all methods and results evaluated and compared for statistical significance. From all methods evaluated, ICC-PCR showed to be the most suitable for virus surveillance in sludge and reclaimed water.
 
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Release Date
2017-03-06
Publishing Date
2013-02-08
 
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