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Doctoral Thesis
DOI
10.11606/T.42.2009.tde-10022010-092618
Document
Author
Full name
Lílian Sauer Albertini
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Rivera, Irma Nelly Gutierrez (President)
Huenuman, Nilton Erbet Lincopan
Matte, Glavur Rogerio
Matte, Maria Helena
Sanchez, Petra Sanchez
Title in Portuguese
Ecologia, fatores associados à virulência e diversidade de Escherichia coli isolados de amostras de água de lastro, água de regiões portuárias e moluscos bivalves no Brasil.
Keywords in Portuguese
Escherichia coli
Água
Antibióticos (Resistência)
ERiC - PCR
Lastro
Microbiologia
Plasmídeos
Virulência (Fatores)
Abstract in Portuguese
Escherichia coli foi isolado de amostras de água de lastro, água de regiões portuárias e de bivalves. 49,6% (164/331) apresentaram múltipla resistência variando de 2 a 8 antibióticos. Dos sete fatores associados à virulência pesquisados: Toxina termoestável (ST), Toxina termolábil (LT), Adesão agregativa (EAEC), Fator de invasão (INV), Toxina Shiga-like I (stx-1), Toxina Shiga-like II (stx-2), e o gene intimina (eae): 4 isolados continham genes homólogos para EAEC, 3 para eae, 3 para ST e uma para stx-2. Um total de 80,0% (24/30), 72,3% (68/94) e 75,3% (55/73) dos isolados de E. coli de amostras de água de lastro, água de regiões portuárias e moluscos bivalves apresentaram plasmídeos, respectivamente. O método ERIC-PCR apresentou melhor desempenho para a análise de agrupamentos. Os isolados de E. coli, com as características encontradas, nos permitirá avaliar o perigo de sua presença no ambiente marinho costeiro e na água de lastro e programas de vigilância sanitária devem ser implementadas para proteger a saúde humana, animal e do ecossistema marinho.
Title in English
Ecology, virulence factors and diversity of Escherichia coli isolated from ballast water, ports areas and bivalves samples in Brazil.
Keywords in English
Escherichia coli
Antibiotics (Resistance)
Ballast
ERiC - PCR
Microbiology
Plasmids
Virulence (factors)
Water
Abstract in English
Escherichia coli was isolated from ballast water, port areas water and bivalves samples. 49.6% (164/331) had multiple antibiotics resistant varied from 2 to 8 antibiotics. From seven virulence associated factors investigated: heat stable toxin (ST), heat labile toxin (LT), aggregative adhesion (EAEC), invasion factor (INV), Shiga-like I toxin (STx-1), Shiga-like II toxin (STx-2), and the gene that codify for intimin (eae): 4 isolates had homology to the EAEC, 3 for eae gene, 3 for ST and one for stx2. A total of 80.0% (24/30), 72.3% (68/94) and 75.3% (55/73) of E. coli isolates from ballast water, port area water and bivalves samples had plasmids, respectively. The ERIC-PCR was more efficiency to analyze the groups. The presence of E. coli isolates with the characteristics found will allow evaluate the hazard present at the coast area ecosystem and ballast water samples and sanitary surveillance programs must be implemented for human, animal and aquatic ecosystem health protection.
 
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Publishing Date
2010-03-23
 
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