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Doctoral Thesis
DOI
Document
Author
Full name
Helena Gabriela Turano
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2017
Supervisor
Committee
Huenuman, Nilton Erbet Lincopan (President)
Gales, Ana Cristina
Ishida, Kelly
Levin, Anna Sara Shafferman
Soares Netto, Luis Eduardo
Title in Portuguese
Avaliação da atividade bactericida da piocina S8 contra cepas de Pseudomonas aeruginosa multirresistentes.
Keywords in Portuguese
Pseudomonas aeruginosa
Bacteriocina
Multirresistente
Piocina S8
Abstract in Portuguese
Pseudomonas aeruginosa é um patógeno oportunista que ocasiona diferentes infecções em humanos. O surgimento de linhagens multirresistentes (MRs), contra os antibióticos comercialmente disponíveis, tem causado elevados níveis de morbidade e mortalidade. O objetivo deste estudo foi identificar uma molécula de piocina que apresente potente atividade bactericida contra cepas de P. aeruginosa MRs. Uma piocina de baixo peso molecular, produzida pela cepa ET02, apresentou potente atividade bactericida contra treze linhagens de P. aeruginosa produtoras de -lactamases (SPM-1, GIM-1, VIM-1, IMP-1, KPC-2, OXA-18 e GES-5). Essa piocina foi purificada e identificada como S8 através de espectrometria de massas e sequenciamento de DNA. Os genes codificadores da piocina S8 estão presentes no interior de um transposon localizado no cromossomo bacteriano da cepa ET02. Estes resultados demonstram que S8 possui potente atividade bactericida contra linhagens de P. aeruginosa MRs, podendo vir a ser utilizada como um composto antimicrobiano no tratamento de infecções hospitalares.
Title in English
Evaluation of the bactericidal activity of the pyocin S8 against multiresistant Pseudomonas aeruginosa strains.
Keywords in English
Pseudomonas aeruginosa
Bacteriocin
Multiresistant
Pyocin S8
Abstract in English
Pseudomonas aeruginosa is an opportunist pathogen that causes human infections. The emergence of multidrug resistant strains (MRs) against antibiotics commercially available has been causing elevated mortality and morbidity levels. The purpose of this study was to identify a molecule of pyocin with a potent bactericidal activity against P. aeruginosa MRs strains. A low molecular weight pyocin, produced by ET02 strain, has presented wide bactericidal activity against thirteen lineages of P. aeruginosa, producers of -lactamases (SPM-1, GIM-1, VIM-1, IMP-1, KPC-2, OXA-18 e GES-5). This pyocin was purified and identified as S8 through mass spectrometry and DNA sequencing. The pyocin S8 encoding genes are present in the interior of a transposon located in the bacterial chromosome of the ET02 strain. These results demonstrate that S8 have potent bactericidal activity against P. aeruginosa MRs lineages, and may well be used as an antimicrobial compound on treatment of hospital infections.
 
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Release Date
2019-11-14
Publishing Date
2017-11-14
 
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