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Doctoral Thesis
DOI
10.11606/T.42.2013.tde-19062013-082012
Document
Author
Full name
Sílvia Regina Loureiro Teixeira
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2013
Supervisor
Committee
Mayer, Marcia Pinto Alves (President)
Feres, Magda
Marques, Marilis do Valle
Piazza, Roxane Maria Fontes
Spolidorio, Denise Madalena Palomari
Title in Portuguese
Análise da variabilidade de sistema de regulação de dois componentes FimSR e expressão do operon fimA em Porphyromonas gingivalis.
Keywords in Portuguese
Porphyromonas gingivalis
Expressão gênica
Microbiologia oral
Polimorfismo
Abstract in Portuguese
O objetivo do presente estudo foi testar a hipótese de que polimorfismo na região que codifica o sistema de dois componentes FimSR e seus níveis de transcrição influenciaria expressão de fímbrias e a capacidade de adesão de Porphyromonas gingivalis. Foram avaliadas 21 cepas clínicas e as cepas de referência 33277 (fimbriada) e W83 (não fimbriada). A presença de cápsula foi determinada em 13/21 cepas e de fímbrias em 15/21. Todas as cepas foram capazes de aderir às células (eficiência de 1,2 a 6,1%). Não houve correlação entre os genótipos fimA, presença de fímbrias / cápsula e perfis de macrorestrição por PFGE. Diferenças na transcrição de fimA não podem ser atribuídas a diferenças na região promotora de fimSR. fimA foi regulado positivamente após interação com células epiteliais na maioria das cepas. Os dados indicam que a regulação de fimA é cepa específica. Cepas não-fimbriadas podem apresentar outras estratégias para aderir às células, sugerindo que, além das fímbrias, outras estruturas poderiam desempenhar um papel na interação dessa espécie com as células.
Title in English
Variability of the two components system FimSR and expression of fimA in Porphyromonas gingivalis.
Keywords in English
Porphyromonas gingivalis
Gene expression
Oral microbiology
Polymorphism
Abstract in English
The aim of this study was to test the hypothesis that polymorphism in genes encoding the two-component system FimSR and their transcription levels would influence the expression of fimbriae and adhesion of Porphyromonas gingivalis. We evaluated 21 clinical strains and reference strains 33277 (fimbriate) and W83 (non fimbriated). The presence of a capsule was determined in 13/21 strains and the presence of fimbriae in 15/21. All strains were able to adhere to the cells (efficiency from 1.2 to 6.1%). There was no correlation between genotypes fimA, presence of fimbriae / capsule and macrorestriction profiles by PFGE. Differences in transcription of fimA could not be attributed to differences in the promoter region of fimS. fimA was upregulated after interaction with epithelial cells in most strains. The data indicate that regulation of fimA is strain specific. Non-fimbriated strains may have other strategies to adhere to epithelial cells, suggesting that in addition to fimbriae, other structures could play a role in the interaction of that specie with cells.
 
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Publishing Date
2013-10-02
 
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