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Master's Dissertation
DOI
10.11606/D.42.2013.tde-24052014-124758
Document
Author
Full name
Anderson Fernandes de Brito
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2013
Supervisor
Committee
Zanotto, Paolo Marinho de Andrade (President)
Setubal, João Carlos
Souza, Robson Francisco de
Title in Portuguese
Análise da diversidade genômica de isolados geográficos do nucleopoliedrovírus de Anticarsia gemmatalis (AgMNPV).
Keywords in Portuguese
Baculoviridae
Evolução molecular
Genômica
Sequenciamento genético
Vírus dos insetos
Abstract in Portuguese
O Anticarsia gemmatalis multiple nucleopolyhedrovirus é usado como agente de controle biológico da lagarta-da-soja (Anticarsia gemmatalis), e representa o bioinseticida viral mais amplamente utilizado no mundo. Sabendo-se que as populações selvagens de baculovírus são geneticamente heterogêneas, este trabalho avaliou a diversidade genômica de 17 isolados selvagens de AgMNPV, coletados no Brasil, Argentina e Uruguai, entre 1980 e 1990. Os genótipos reconstruídos evidenciam a conservação da colinearidade genômica durante a evolução de AgMNPV, porém, deleções e inserções de uma ou mais bases foram comuns. Um novo gene com possível origem em lepidóptero foi identificado em dois genótipos; e o gene bro-a está ausente em muitos isolados. Análises de diversidade genética apontaram que a maioria dos genes de AgMNPV é pouco variável, e os genes mais diversos estão em loci variáveis específicos. Os resultados obtidos reforçam a percepção de que grandes vírus de DNA evoluem principalmente sob baixas taxas de substituição, e por meio de duplicações, ganhos e perdas gênicas.
Title in English
Genomic diversity analysis of geographic isolates of nucleopolyhedroviruses Anticarsia gemmatalis (AgMNPV).
Keywords in English
Baculoviridae
Genetic sequencing
Genomics
Molecular evolution
Viruses of insects
Abstract in English
The Anticarsia gemmatalis multiple nucleopolyhedrovirus is employed as a biological control agent against velvetbean caterpillar (Anticarsia gemmatalis), and represents the most widely used viral bioinseticide in the world. Since baculovirus wild populations are genetically heterogeneous, this work evaluated the genomic diversity of 17 AgMNPV geographic isolates obtained from 1980 to 1990, in the Brazil, Argentina and Uruguay. The genotypes reconstructed evidenced the highly genomic colinearity conservation during AgMNPV evolutionary history, however, deletions and insertions of one or more bases were common. A new gene with possible lepidopteran origin was identified in two genotypes; and the gene bro-a is absence in many isolates. Genetic diversity analyses pointed that most AgMNPV genes shows little variation, and the highly diverse genes are in specific variable loci. The obtained results reinforce the perception that large DNA virus evolve mainly under low rates of substitution, and through gene duplications, gain and loss.
 
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Publishing Date
2014-05-28
 
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