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Doctoral Thesis
DOI
10.11606/T.42.2009.tde-28102009-094653
Document
Author
Full name
Franklin Geronimo Bispo Santos
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Guimarães, Elizabeth Oliveira da Costa Freitas (President)
Benites, Nilson Roberti
Birgel Junior, Eduardo Harry
Vicente, Elisabete Jose
Zafalon, Luiz Francisco
Title in Portuguese
Estudo epidemiólogico-molecular e de fatores de virulência de Staphylococcus aureus associados à mastite bovina em propriedades de exploração leiteira dos Estados de São Paulo e Pernambuco.
Keywords in Portuguese
Staphylococcus aureus
Biofilmes
Epidemiologia molecular
Mastite bovina
Microbiologia
PFGE e PCR (Reação em cadeia por polímerase)
Portador de doença animal
Abstract in Portuguese
Um total de 107 S. aureus isolados de casos de mastite, glândulas portadoras, pele do úbere, ordenhadores e insufladores, em rebanhos de São Paulo e Pernambuco, foram tipados por técnicas moleculares PCR-RFLP do gene coa e PCR de spa distinguiram seis perfis. Todas as amostras amplificaram genes coa, spa, icaA e 69% produziram biofilme glicose-induzido in vitro. PFGE identificou 31 perfis e 12 linhagens. Uma linhagem foi predominante (P < 0,0001) e amplamente disseminada em ambas as regiões. Um mesmo perfil foi isolado de mastite clínica, subclínica e portadoras. Houve heterogeneidade genética entre isolados de fazenda. Isolados de origem humana e animal constituíram populações distintas. Poucos isolados de leite, insufladores e pele do úbere tiveram igual perfil. Uma amostra extramamária, 77% dos isolados de leite e. 99% de S. aureus de portadoras produziram biofilme. Não foi detectada correlação entre produção de biofilme e CCS. O isolamento sucessivo do mesmo perfil de PFGE de glândulas assintomáticas por mais de 16 dias caracterizou o estágio de portador.
Title in English
Molecular epidemiology and virulence factors of Staphylococcus aureus associated to bovine mastitis in dairy herds from São Paulo and Pernambuco state.
Keywords in English
Staphylococcus aureus
Biofilms
Bovine mastitis
Carrier status
Microbiology
Molecular epidemiology
PFGE and PCR (Polymerase Chain Reaction)
Abstract in English
A total of 107 S. aureus isolated from bovine milk, udder skin, milkers and milking machine, from São Paulo and Pernambuco herds was typed by molecular techniques. PCR-RFLP coa gene and PCR spa gene distinguished six amplicons. All strains amplified coa, spa, icaA genes and, 69% produced in vitro glucose-induced biofilm. PFGE identified 31 pulsotypes, 12 lineages. One of the lineages was predominantly isolated (P<0.0001) and widely disseminated. A same pulsotype was isolated from clinical and, subclinical mastitis as well as from carriers. There was genetic heterogeneity among isolates from the herds. Strains from human and animal origin were genetically different. Few isolates from milk, milking machine and udder skin showed similar pulsotype. An extramammary strain, 77% of the milk isolates and, 99% of the S. aureus isolated from carriers produced biofilm. It was not detected any correlation between SCC and biofilm production. The successive isolation during more than 16 days of a same pulsotype from the asymptomatic glands characterized the carrier status.
 
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Publishing Date
2009-12-04
 
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