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Master's Dissertation
DOI
10.11606/D.42.2010.tde-11012011-153707
Document
Author
Full name
Lilian Sally Chin
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Barbuto, Jose Alexandre Marzagao (President)
Fecchio, Denise
Lepique, Ana Paula
Title in Portuguese
Avaliação da ativação linfócitária por diferentes combinações de células apresentadoras de antígenos.
Keywords in Portuguese
Células cultivadas de tumor
Células dendríticas
Citocinas
Imunologia celular
Linfócitos T
Proliferação celular
Abstract in Portuguese
Acredita-se que as células dendríticas (DCs) sejam as mais eficientes na ativação de linfócitos T (LT) naive. Com a possibilidade de geração in vitro de DCs, muitos protocolos explorando este potencial vêm sendo desenvolvidos, principalmente em abordagens imunoterapêuticas para o câncer. Todavia, em situações fisiológicas a apresentação antigênica dificilmente ocorre por um tipo celular único. Deste modo, este trabalho investigou os padrões de reposta de LT induzidos por DCs maduras (mDCs) em combinação com diferentes APCs, incluindo linfócitos B, monócitos, macrófagos e DCs imaturas. Os padrões de resposta gerados pelos LT foram analisados por citometria de fluxo, ELISA e BIOPLEX. A estimulação dos LT pelas mDCs isoladas apresentaram o maior poder de estimulação, seguidas pelas outras APCs. Todas as combinações diminuiram a resposta induzida pelas mDCs, principalmente de LT CD4+. Deste modo, os dados confirmam o efeito das interações de APCs na estimulação de LT provendo ferramentas para o refinamento de abordagens imunoterapêuticas.
Title in English
Evaluation of lymphocyte activation by different combinations of antigen presenting cells.
Keywords in English
Cell proliferation
Cellular immunology
Cultured tumor cells
Cytokines
Dendritic cells
T lymphocytes
Abstract in English
Dendritic cells (DC) are the main APC able to activate T cells (TC). Since they may be generated in vitro, many protocols based on their immunostimulatory potential are currently underway, mainly in immunotherapeutic approaches for cancer. In physiological conditions, however, other APC may participate in antigen presentation, thus influencing the immune response pattern developed. Therefore, the aim of this study was to evaluate the TC response patterns induced in vitro by mature DC (mDC) combined with different APC, including B cells, monocytes, macrophages and immature DC. The response patterns were analyzed flow cytometry, ELISA and Multiplex flow immunoassay. The TC stimulation by isolated mDC presented the highest capacity of stimulation, followed by the other APC. All combinations decreased the response induced by mDC, mainly CD4+ T cells. These data confirm the effects of APC interactions upon TC stimulation and may provide a tool for fine-tuning of immune response induction in immunotherapeutic approaches.
 
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Publishing Date
2011-05-16
 
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