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Master's Dissertation
DOI
10.11606/D.42.2014.tde-14032015-090306
Document
Author
Full name
Bianca Balbino
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Russo, Momtchilo (President)
Basso, Alexandre Salgado
Faria, Ana Maria Caetano de
Title in Portuguese
Tolerância cruzada no modelo de inflamação pulmonar alérgica experimental.
Keywords in Portuguese
Blomia tropicalis (Bt)
Asma alérgica experimental
Hemocianina de Keyhole limpet (KLH)
Ovalbulmina (OVA)
Tolerância cruzada
Abstract in Portuguese
A tolerância pode ser considerada um dos pilares da imunologia. Sabe-se que a tolerância a um antígeno pode gerar tolerância a outro antígeno não relacionado, fenômeno conhecido como tolerância cruzada. Neste trabalho caracterizamos a tolerância cruzada utilizando a OVA como tolerógeno e extrato de Blomia tropicalis (Bt) ou Hemocianina de Keyhole limpet (KLH) como alérgenos. Verificamos que é possível reproduzir o fenômeno da tolerância cruzada neste modelo de inflamação alérgica induzida tanto pelo KLH quanto pela Bt, com diminuição do infiltrado inflamatório no pulmão, eosinófilos, IgE total e produção de muco. Ainda, a estratégia de utilizar a tolerância cruzada terapeuticamente, i.é., após a sensibilização com KLH, a indução de tolerância cruzada não foi capaz de prevenir a resposta alérgica. Em conjunto, nossos dados mostram que a tolerância à OVA modifica as respostas alérgicas tanto à Bt quanto ao KLH no modelo de inflamação pulmonar experimental de forma profilática, mas que a tolerância cruzada não é eficiente em animais já sensibilizados.
Title in English
Cross-tolerance in a model of experimental allergic lung inflammation.
Keywords in English
Blomia tropicalis (Bt)
Keyhole limpet hemocianina (KLH)
Allergic lung disease
Cross-tolerance
Ovalbumin (OVA)
Abstract in English
Tolerance is among the Immunology pillars. Experimental data indicate that tolerance towards an antigen can promote tolerance to an unrelated antigen, a phenomenon known as cross-tolerance. Here we sought to characterize cross tolerance using OVA as a tolerogen and Blomia tropicalis (Bt) extract or Keyhole limpet Hemocianina (KLH) as allergens. We found that cross tolerance can be reproduced in the model of allergic lung disease induced by KLH or Bt, with less inflammatory infiltrate in the lung, eosinophils, total IgE and mucus production. Using cross tolerance therapeutically, i.e., after KLH sensitization, was not effective, since the allergic lung response was not modulated. Altogether, our data shows that OVA tolerance modulate allergic lung disease induced either by Bt or KLH when used as prophylactic model, however cross tolerance is ineffective in sensitized animals.
 
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Publishing Date
2015-03-14
 
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