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Doctoral Thesis
DOI
10.11606/T.42.2007.tde-18102007-103406
Document
Author
Full name
Claudia Andréa Alves de Araújo
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2007
Supervisor
Committee
Soares, Maria Fernanda de Macedo (President)
Araujo, Maria Ilma Andrade Santos
Goto, Hiro
Negro, Sonia Jancar
Sato, Maria Notomi
Title in Portuguese
Estudos da imunomodulação induzida por PAS-1 (proteína imunossupressora de Ascaris suum) na inflamação alérgica pulmonar.
Keywords in Portuguese
Ascaris suum
Asma
Citocinas
Imunomodulação
Linfócitos T
Abstract in Portuguese
Neste trabalho, investigamos os mecanismos da resposta imune estimulados por PAS-1 para o desencadeamento do seu efeito modulatório na inflamação alérgica pulmonar. Para tanto, camundongos C57BL/6 selvagens, IL-12-/-, IFN-g-/- e IL-10-/- foram imunizados e desafiados com OVA ou PAS-1 ou OVA + PAS-1. Ainda, camundongos C57BL/6 imunizados e desafiados com OVA receberam transferência adotiva de células CD19+, B220+, CD3+, CD4+, CD8+, CD4+CD25-, CD4+CD25+ PAS-1-primadas. Nossos resultados demonstraram que o efeito imunomodulatório de PAS-1 é mediado por IFN-g e IL-10, mas não por IL-12, e que somente camundongos que receberam células CD8+ ou CD4+CD25+ não desenvolveram inflamação pulmonar e produziram, respectivamente, IFN-g e IL-10/TGF-ß. Em conjunto, estes resultados demonstraram que o efeito imunomodulatório de PAS-1 é devido à estimulação de células T CD8+ produtoras de IFN-g e por CD4+CD25+ secretoras de IL-10 e TGF-ß.
Title in English
Studies of the immunomodulation induced by PAS-1 (immunosuppressive protein from Ascaris suum) in the lung allergic inflammation.
Keywords in English
Ascaris suum
Asthma
Cytokines
Immunomodulation
T lymphocytes
Abstract in English
In this work, we investigate the immune response mechanisms triggered by PAS-1 to promote its immunomodulatory effect in the lung allergic inflammation. For that, wild type, IL-12-/-, IFN-g-/- and IL-10-/- C57BL/6 mice were immunized and challenged with OVA or PAS-1 or OVA + PAS-1. Moreover, wild type C57BL/6 mice were adoptively transferred with PAS-1-primed CD19+, B220+, CD3+, CD4+, CD8+, CD4+CD25-, and CD4+CD25+ lymphocytes. Our results demonstrated that the immunomodulatory effect induced by PAS-1 is mediated by IFN-g and IL-10, but not by IL-12, and only mice which received CD8+ or CD4+CD25+ T cells did not present lung allergic inflammation and produced IFN-g and IL-10/TGF-ß, respectively. Taken together, these results demonstrated that the imunomodulatory effect induced by PAS-1 is due to stimulating CD8+ T cells, which secrete IFN-g, and CD4+CD25+ T cells, which secrete IL-10 and TGF-ß.
 
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Publishing Date
2007-10-22
 
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