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Master's Dissertation
DOI
10.11606/D.42.2007.tde-30012008-110420
Document
Author
Full name
Renato Brito Baleeiro
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2007
Supervisor
Committee
Barbuto, Jose Alexandre Marzagao (President)
Capelozzi, Vera Luiza
Mosig, Jose Maria Alvarez
Title in Portuguese
Estudo da expressão de citocinas no microambiente tumoral em pacientes com neoplasias pulmonares e sua correlação com a presença de macrófagos e células dendríticas.
Keywords in Portuguese
Câncer.
Células dentríticas
Citocinas
Imunohistoquímica
Macrófagos
Neoplasias pulmonares
Abstract in Portuguese
Células dendríticas (DCs) são centrais na indução da resposta imune e o desvio de sua diferenciação para macrófagos, no microambiente tumoral pode ser um mecanismo de escape do tumor frente à resposta imune. Assim, o objetivo deste projeto foi comparar, entre tecido neoplásico e não-neoplásico, a freqüência de DCs, macrófagos e mastócitos e da produção in situ das citocinas IL-4 e TNF-a. Nesta análise observou-se maior quantidade de DCs (CD1a+ ou S100+), macrófagos (CD68+), mastócitos (azul de toluidina+) e células produtoras de TNF-a no tumor do que no tecido pulmonar. No tecido pulmonar encontrou-se maior freqüência de células positivas para IL-4, assim como, entre células dissociadas de tecido fresco, observou-se maior freqüência de DCs maduras. Identificou-se também a expressão, por células tumorais ou do estroma pulmonar, do marcador CD83, característico de DCs ativadas, em 10 de 11 pacientes. O sobrenadante de cultura destas células CD83+ inibiu a ativação de linfócitos por DCs alogenêicas, mas esta atividade foi suprimida por anticorpos anti-CD83.
Title in English
Study of the expression of cytokines in the tumor microenvironment in lung cancer patients and their correlation with the presence of macrophages and dendritic cells.
Keywords in English
Cancer.
Cytokines
Dendritic cells
Immunohistochemistry
Lung cancer
Macrophages
Abstract in English
Dendritic cells (DCs) play a crucial role in the immune response and the deviation of its differentiation to macrophages in the tumor microenvironment may be a mechanism of escape of tumor from immune response. Thus, the aim of this project was to compare between affected and non-affected lung, the frequency of DCs, macrophages and mast cells and production in situ of IL-4 and TNF-a. In this analysis, we observe higher amount of DCs (CD1a+ and S-100+), macrophages (CD68+), mast cells (toluidine blue+) and cells producing TNF-a in the tumor than non-affected lung. In lung, we found higher frequency of cells IL-4+ and in lung digests we observed higher amount of mature DCs. We also identified the expression by tumor or stroma cells the CD83, present in activated DCs, in 10 of 11 patients. The supernatant of such cells CD83+ inhibited the activation of lymphocyte by alogeneic DCs, but this effect was decreased by anti-CD83.
 
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Publishing Date
2008-02-07
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • BALEEIRO, R. B., et al. Expression of a dendritic cell maturation marker CD83 on tumor cells from lung cancer patients and several human tumor cell lines: is there a biological meaning behind it? [doi:10.1007/s00262-007-0344-x]. Cancer Immunology, Immunotherapy [online], 2007, vol. 57, n. 2, p. 265-270.
  • BALEEIRO, R. B., et al. High frequency of immature dendritic cells and altered in situ production of interleukin-4 and tumor necrosis factor-α in lung cancer [doi:10.1007/s00262-008-0468-7]. Cancer Immunology, Immunotherapy [online], 2008, vol. 57, n. 9, p. 1335-1345.
  • BALEEIRO, R.B., and BARBUTO, J.A.M.. Local secretion/shedding of tumor-derived CD83 molecules as a novel tumor escape mechanism [doi:10.1016/j.molimm.2008.04.005]. Molecular Immunology [online], 2008, vol. 45, n. 12, p. 3502-3504.
  • TOMIYOSHI, M.Y., et al. Cohabitation with a B16F10 melanoma-bearer cage mate influences behavior and dendritic cell phenotype in mice [doi:10.1016/j.bbi.2009.02.006]. Brain, Behavior, and Immunity [online], 2009, vol. 23, n. 4, p. 558-567.
All rights of the thesis/dissertation are from the authors
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