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Doctoral Thesis
DOI
Document
Author
Full name
Lilian Cruz
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2017
Supervisor
Committee
Lopes, Marilene Hohmuth (President)
Braga, Patricia Cristina Baleeiro Beltrao
Giordano, Ricardo José
Pierulivo, Enrique Mario Boccardo
Yan, Chao Yun Irene
Title in Portuguese
Perfil de miRNAs intracelulares e liberados via vesículas extracelulares na diferenciação neural de células-tronco pluripotentes.
Keywords in Portuguese
Células-tronco pluripotentes
Diferenciação neural
miRNAs
Vesículas extracelulares
Abstract in Portuguese
As células-tronco processam e são sensíveis a múltiplos sinais dentro de seu microambiente, os quais podem exercer influências que regulam seu destino e sua função de forma espaço temporal. Neste contexto, células podem exercer seu papel biológico por transferir informação genética e alterar expressão gênica de alvos celulares através de vesículas extracelulares (VEs). MicroRNAs (miRNAs), uma classe de pequenos RNAs não codificantes, podem ser encontrados nestas vesículas e são considerados moléculas efetivas no controle do neurodesenvolvimento por regular genes chaves em tempo controlado. Pouco se sabe sobre como a diferenciação influencia o conteúdo de miRNAs liberados via VEs revelando o papel dos mesmos no microambiente de cada etapa do comprometimento neural. Assim, a proposta deste estudo foi analisar o perfil de miRNAs intracelulares e presentes em VEs envolvidos na diferenciação neural dopaminérgica de células-tronco pluripotentes e identificar os possíveis alvos regulados pelos mesmos como mecanismo de estabelecimento de um destino neural específico.
Title in English
Intracellular and extracellular vesicles miRNAs profile during neural differentiation of pluripotent stem cells.
Keywords in English
Extracellular vesicles
miRNAs
Neural differentiation
Pluripotent stem cells
Abstract in English
Stem cells sense and process multiple signals in their microenvironment, which can exert influences that regulate cell fate and function in a time spatial manner. In this context, the stem cells can exert their biological role transferring genetic information and altering the genetic expression of target cells through extracellular vesicles (EVs). MicroRNAs (miRNAs), a class of small non coding RNAs, can be found in those EVs and are considered effective molecules in the control of neurodevelopment and differentiation by regulating key genes in a time specific manner. However, little is known about how the cell differentiation influences the miRNAs content released through EVs, and how these molecules function in the microenvironment of each phase of neural commitment. Thus, the purpose of this study was to analyze the intracellular and EVs miRNAs profiles involved in the dopaminergic differentiation of pluripotent stem cells in attempt to identify possible targets regulated by miRNAs as a mechanism of specific neural fate decision.
 
There are withheld file due to requirements (data publishing, patents or rights).
Release Date
2021-07-07
Publishing Date
2017-07-07
 
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