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Doctoral Thesis
DOI
10.11606/T.42.2015.tde-07122015-195142
Document
Author
Full name
Rodrigo Barbano Weingrill
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2015
Supervisor
Committee
Bevilacqua, Estela Maris Andrade Forell (President)
Barboza, Renato
Freitas, Vanessa Morais
Oliveira, Sergio Ferreira de
Palmeira, Patricia
Title in Portuguese
Expressão da quimiocina Ccl25 e seu receptor Ccr9 no processo de implantação embrionária em camundongos.
Keywords in Portuguese
Blastocisto
Ccl25
Ccr9
Cone ectoplacentário
Implantação embrionária
Quimiocinas
Abstract in Portuguese
Neste estudo foi analisada a expressão gênica e protéica, uterina e embrionária da quimiocina Ccl25 e de seu receptor Ccr9 nas fases iniciais da implantação embrionária (dias 3,5, 4,5, 5,5 e 7,5 de gestação). Por meio de reações imunohistoquímicas e de citometria de fluxo, foram identificadas as populações celulares envolvidas nesta expressão. Também, foram realizados ensaios de quimiotaxia com o silenciamento da expressão de Ccl25 (ODNs-Antisense) nas células trofoblásticas, para a avaliação das atividades desta quimocina. Nossos resultados sugerem o estabelecimento de uma comunicação embrião (células trofoblásticas) - endométrio (células do sistema immunológico), via Ccl25/Ccr9. Esses achados são relevantes para a compreensão das interações blastocisto/sistema immunológico materno no estabelecimento dos mecanismos imunoreguladores durante a implantação embrionária.
Title in English
Expression of chemokine (C-C) motif 25 and its receptor during mouse embryo implantation.
Keywords in English
Blastocyst
Ccl25
Ccr9
Chemokines
Ectoplacental cone
Embryo implantation
Abstract in English
In this study, we analyzed the gene and protein, uterine and embryonic expression of Ccl25 chemokine and its receptor Ccr9 in early stages of embryo implantation (days 3.5, 4.5, 5.5 and 7.5 of gestation). By using immunohistochemistry and flow cytometric assays, cell populations involved in this expression were identified. In addition, chemotaxis assays were also performed after silencing of Ccl25 (ODNs-antisense) in trophoblast cells. . Our results suggest the establishment of an endometrium (immune cells) - embryo (trophoblast cells) dialogue via Ccl25/CCR9. These findings are relevant for understanding the interactions between blastocyst and maternal immune system in the establishment of immunoregulatory mechanisms during implantation.
 
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Release Date
2017-12-06
Publishing Date
2015-12-07
 
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