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Master's Dissertation
DOI
10.11606/D.42.2017.tde-10052017-134839
Document
Author
Full name
Caroline Borgato Guedes
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2017
Supervisor
Committee
Bevilacqua, Estela Maris Andrade Forell (President)
Borrelli, Giuliano Moysés
Rosa Neto, José Cesar
Title in Portuguese
O soro de mulheres com endometriose altera os níveis de citocinas produzidas pelas células estromais e endoteliais uterinas cocultivadas em sistema 3D.
Keywords in Portuguese
Citocinas
Cocultura
Cultura 3D
Endométrio
Endometriose
Abstract in Portuguese
A endometriose é caracterizada pela presença de tecido endometrial fora do útero. No estudo, utilizou-se um sistema de co-cultivo 3D contendo células do estroma e endotélio endometrial. O sistema foi exposto ao soro de mulheres saudáveis ou com endometriose, que fazem ou não o uso de anticoncepcional. Posteriormente, foi avaliada a resposta do sistema no que diz respeito ao perfil de citocinas. Nossos resultados mostraram que o perfil sérico das mulheres com endometriose sem anticoncepcional apresentaram elevados níveis séricos de IL2 e IL10 com relação às saudáveis. Enquanto que as mulheres com endometriose, que fazem uso de anticoncepcional, apresentaram altos níveis de IL6 e IL8. O perfil de citocinas encontrado no homogenato das mulheres com endometriose induziu a produção exacerbada de IL2 e IL10 além de IFN-γ, TNF-α, IL6. Quando as células eram incubadas com soro de mulheres com endometriose que fazem tratamento, os níveis de IL6 e IL8 aumentaram. Os achados sugerem que o uso de co-cultivos 3D de células estromais e endoteliais endometriais é um bom modelo para novos estudos.
Title in English
Serum from women with endometriosis altering the levels of cytokines produced by stromal and endothelial endometrial cells in 3D coculture system.
Keywords in English
3D culture
Co-culture
Cytokines
Endometriosis
Endometrium
Abstract in English
Endometriosis is characterized by the presence of endometrial tissue outside the uterus. In this study, we used a 3D co-culture system with stromal and endothelium endometrial cells. The system was exposed to serum of healthy women or with endometriosis do or not use contraceptive. Subsequently, he studied the response of endometrial cells with respect to the cytokine profile. Our results showed that the serum profile of women with endometriosis without contraceptive had high serum levels of IL2 and IL10 regarding healthy women. While women with endometriosis who use contraceptive, showed high levels of IL6 and IL8. The cytokine profile seen in homogenate of women with endometriosis induced high production of IL2 and IL10, in addition IFN-γ, TNF-α, IL6. When cells were incubated with serum from women with endometriosis under treatment, the levels of IL6 and IL8 increased. The findings suggest that the use of co-cultivation 3D stromal cells and endometrial endothelial is a good model for further studies.
 
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Release Date
2019-05-10
Publishing Date
2017-05-10
 
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