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Master's Dissertation
DOI
10.11606/D.42.2010.tde-16122010-150034
Document
Author
Full name
Chayrra Chehade Gomes
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Borella, Maria Ines (President)
Batlouni, Sérgio Ricardo
Jaeger, Ruy Gastaldoni
Title in Portuguese
Localização das diferentes formas de GnRH no encefálo de Astyanax altiparanae (Garutti e Britski, 2000) e Danio rerio (Hamilton, 1822).
Keywords in Portuguese
Astyanax altiparanae
Danio rerio
Encefalopatia animal
Esteróides
Glândula pituitária
GnRH1
GnRH3
Imuno-histoquímica
Peixes de água doce
Peptídeos
Reprodução
Abstract in Portuguese
O GnRH é um decapeptídeo que está envolvido na reprodução, estimulando a hipófise a liberar gonadotropinas (LH e FSH), as quais regulam a esteroidogênese e gametogênese. Ainda, o GnRH age como neuromodulador atuando no comportamento sexual. A distribuição de suas isoformas pode ajudar a revelar a função específica de cada GnRH. A principal ênfase deste estudo foi detectar a presença e distribuição por imuno-histoquímica, e a expressão gênica de diferentes formas de GnRH no encéfalo de Astyanax altiparanae e Danio rerio, os quais têm importância comercial, ecológica e acadêmica. Em Astyanax altiparanae foi encontrado GnRH3 em diversos corpos celulares, inclusive em corpos celulares ligados à função reprodutiva, juntamente com fibras que inervam a neuro-hipófise. O GnRH1 foi encontrado apenas em fibras. Em Danio rerio foi encontrado GnRH3 nos núcleos da região hipotalâmica e em um grande número de fibras, incluindo as que inervam a neuro-hipófise. A expressão gênica de GnRH2 e 3 foi observada em Danio rerio.
Title in English
Localization of different forms of GnRH in the brain of Astyanax altiparanae (Garutti and Britski, 2000) and Danio rerio (Hamilton, 1822).
Keywords in English
Astyanax altiparanae
Danio rerio
Animal encephalopathy
Freshwater fish
GnRH1
GnRH3
Immunohistochemical technique
Peptides
Pituitary gland
Reproduction
Steroids
Abstract in English
GnRH is a decapeptide involved in reproduction, stimulating the pituitary to release gonadotropins, which, in turn, regulate the steroidogenesis and gametogenesis. In addition to the reproductive function, the GnRH displays neuromodulatory roles with implications in the modulation of sexual behavior. Therefore, the main emphasis of this study is to detect the presence, distribution, and gene expression of different forms of GnRH in the brain of the freshwater teleosts Astyanax altiparanae and Danio rerio, which have commercial, ecological and academic importance. The immunohistochemical method of peroxidase was used to detect GnRHs in the brain and pituitary. In A. altiparanae immunoreactivity to anti-GnRH3 was found in various cell bodies, including those related to reproductive functions, and fibers which innervate the neurohypophysis. Immunoreactivity for GnRH1 was found only in fibers. In D. rerio immunoreactivity to anti-GnRH3 was found in hypothalamic nuclei and in a large number of fibers, including the ones which innervate the neurohypophysis.
 
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Publishing Date
2011-03-02
 
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