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Master's Dissertation
DOI
10.11606/D.42.2007.tde-18102007-100214
Document
Author
Full name
Cãmila Valentim Deluca
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2007
Supervisor
Committee
Moriscot, Anselmo Sigari (President)
Brum, Patricia Chakur
Yan, Chao Yun Irene
Title in Portuguese
Efeito da sobrecarga de leucina no músculo sóleo de ratos durante a distração gradual osteogênica.
Keywords in Portuguese
Dano muscular
Distração gradual osteogênica
Leucina
Músculo esquelético
Sarcomerogênese
Trofismo muscular
Abstract in Portuguese
O objetivo deste estudo foi investigar o efeito da sobrecarga de leucina no músculo sóleo de ratos submetidos à DGO (Distração Gradual Osteogênica). Vinte e cinco ratos Wistar foram divididos em 5 grupos: controle, sham-lengthening, leucina, DGO e DGO+leucina. A DGO de 2mm/dia foi realizada na tíbia até um ganho de 20% e a sobrecarga de leucina (114mM) foi administrada oralmente. Avaliou-se: estrutura histológica muscular, número de sarcômeros em série, área dos diferentes tipos de fibras musculares e análise do tecido conjuntivo. Os dados obtidos dos cortes histológicos mostram que a DGO promoveu intensa lesão muscular e aumento do tecido conjuntivo, o que foi minimizado pela leucina. Houve uma adição no número de sarcômeros em série (26%) durante a DGO, que foi intensificada pela leucina (33%). Observou-se uma diminuição da área de todos os tipos de fibras com a DGO e um aumento da área das fibras do tipo I com a leucina. Portanto, a leucina minimizou os efeitos causados pela DGO, podendo esta ser utilizada como um agente terapêutico durante a DGO no futuro.
Title in English
Effect of the leucine overload in rat soleus muscle during gradual distraction osteogenesis.
Keywords in English
Gradual distraction osteogenesis
Leucine
Muscle injury
Muscle trophism
Sarcomerogenesis
Skeletal muscle
Abstract in English
The goal of the present study was to investigate the effect of leucine overload in the rat soleus muscle before and along DGO (Gradual Distraction Osteogenesis). Twenty five male Wistar rats were divided into 5 groups: control, sham-lengthening, leucine, DGO and DGO+leucine. The DGO was 2mm/day and the leucine overload (114mM) was administrated orally. Subsequently analyzed: histological cross-section, sarcomere serial number and cross-sectional area of the types fibers and connective tissue. The histological data showed that the DGO promoted intense injury and increase of connective tissue, which can be minimized by leucine. The sarcomere serial number increased by ~26% and leucine overload increased this gain to 33%. The fibers types showed a decrease of the cross-section area of the all type fibers with the DGO and increase of the type I fibers area with leucine overload. We conclude that leucine overload is a potential therapeutical device in DGO, seeking improvement of skeletal muscle longitudinal growth.
 
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Publishing Date
2007-10-24
 
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