• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Master's Dissertation
DOI
10.11606/D.42.2014.tde-19022015-164340
Document
Author
Full name
Suély Vieira da Silva
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Freitas, Vanessa Morais (President)
Borella, Maria Ines
Cardoso, Alexandre Bruni
Title in Portuguese
Influência do estrógeno, progesterona e testosterona nos níveis de expressão e na distribuição de ADAMTS-1 (uma desintegrina e metaloproteinase com domínios trombospondinas 1) em células mamárias humanas normais e tumorais.
Keywords in Portuguese
ADAMTS-1
Câncer de mama
Hormônios
Matriz extracelular
Metaloproteinases da matriz
Núcleo
Abstract in Portuguese
O câncer de mama é no Brasil o segundo maior causador de morte entre mulheres. Os hormônios sexuais estão dentre os vários fatores indutores ou promotores da carcinogênese. ADAMTS (uma desintegrina e metaloproteinase com domínios trombospondina) é uma enzima Zn2+/Ca2+dependente. Avaliamos a influência dos hormônios na expressão e localização da ADAMTS-1 em 3 linhagens de mama. qPCR demonstrou que a progesterona estimulou o aumento de mRNA de ADAMTS-1 na MCF-10A e na MDA-MB-231, o tratamento com estrógeno e progesterona estimulou a diminuição. No Western blot observamos nas linhagens MCF-10A e MCF-7 que os hormônios tendem a aumentar os níveis de ADAMTS-1, e o estrógeno estimulou uma acentuada secreção em MCF-7. Na linhagem MDA-MB-231, os tratamentos demonstraram uma tendência a diminuir os níveis de ADAMTS-1 intracelular. Na imunofluorescência e Western blot observamos a predominância de ADAMTS-1 nuclear. Os resultados sugerem que os hormônios regulam a expressão de ADAMTS-1 nas células normais e tumorais, e que está predominantemente presente no núcleo celular.
Title in English
Influence of estrogen, progesterone and testosterone on ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin motifs 1) levels and distribution in normal and tumoral breast cells.
Keywords in English
ADAMTS-1
Breast câncer
Extracellular matrix
Hormones
Matrix metalloproteinases
Nucleus
Abstract in English
Breast cancer is the second largest cause of death among women in Brazil. Sex hormones are one of the several inducers factors or cancer promoters. ADAMTS (a disintegrin and metaloproteinase with thrombospondin motifs) are dependent on Zn2+/Ca2+ enzymes. We evaluated influence of hormones on ADAMTS-1 levels and localization in three different breast cell lines. qPCR showed that progesterone stimulated an increase of ADAMTS-1 mRNA in MCF-10A, however, in MDA-MB-231, the treatment by estrogen and progesterone decreased levels. Western blot analysis showed a tendency to increased ADAMTS-1 levels in MCF-10A and MCF-7 due to the hormone treatment. Treatment by estrogen led to a marked secretion in MCF-7. In MDA-MB-231 the treatment showed a tendency to decreased intracellular ADAMTS-1 protein levels. Immunofluorescence and Western Blot we observed ADAMTS-1 predominant in the nucleus. Results suggest that sex hormones regulate the ADAMTS-1 expression in normal and tumoral breast cells, ADAMTS-1 is predominant in the cellular nucleus.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Release Date
2017-02-19
Publishing Date
2015-02-20
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
Centro de Informática de São Carlos
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2021. All rights reserved.